Reversed phase chromatography polymeric media

QC Wang, F Svec, MJ Frechet. Macroporous polymeric stationary-phase rod as continuous separation medium for reversed-phase chromatography. Anal Chem 65 2243-2248, 1993. 

Q. C. Wang, F. Svec, and J. M. J. Frechet, Macroporous Polymeric Stationary Phase Rod as Continuous separation Medium for Reversed-Phase Chromatography, Anal. Chem., 65 2243-2248 (1993). 

In reversed phase liquid chromatography (RPLC) silylated silicas are preferred. The surface of these silicas is covered with chemically bonded non-polar groups such as alkyl chains or polymeric layers (Chapter 3.2.3). Silica modified with medium polar groups such as cyano, diol or amino might be used in NP as well as RP mode. Alternatively, cross-linked polymers such as hydrophobic styrene divinyl benzene-copolymers can be used (Chapter 3.2.4). Polymer packings show stability in a pH range 2-14 while silica based packings show limited stability for pH > 7. 

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