Reverse-phase high-performance liquid separation

W. G. Button, K. D. Nugent, T. K. Slattery, B. R. Summers and L. R. Snyder, Separation of proteins by reversed-phase high-performance liquid chromatography , 7. Chromatogr. 443 363-379(1988). 

Baillet, A., Corbeau, L., Rafidson, R, and Ferrier, D., Separation of isomeric compounds by reversed-phase high-performance liquid chromatography using Ag+ complexation. Application to cis-trans fatty acid methyl esters and retinoic acid photoisomers, /. Chromatogr., 634, 251, 1993. 

Stadalius, M. A., Gold, H. S., and Snyder, L. R., Optimization model for the gradient elution separation of peptide mixtures by reversed-phase high-performance liquid chromatography. Verification of retention relationships, /. Chromatogr., 296, 31, 1984. 

Koyama, J., Nomura, J., Shiojima, Y., Ohtsu, Y., and Horii, I., Effect of column length and elution mechanism on the separation of proteins by reversed-phase high-performance liquid chromatography, /. Chromatogr., 625, 217, 1992. 

Jandera, P., Correlation of retention and selectivity of separation in reversed-phase high-performance liquid chromatography with interaction indices and with lipophilic and polar structural indices, J. Chromatogr. A, 656, 437, 1993. 

Terabe, S., Nishi, H., and Ando, T., Separation of cytochromes c by reversed-phase high-performance liquid chromatography, J. Chromatogr., 212, 295,1981. 

Hayakawa, K., Hirano, M., Yoshikawa, K., Katsumata, N, and Tanaka, T., Separation of phenylthiohydantoin-amino acids by temperature-controlled reversed-phase high-performance liquid chromatography, /. Chromatogr. A, 846, 73, 1999. 

Wall, D. B Kachman, M. T Gong, S. Y. S Parus, S. J Long, M. W Lubman, D. M. (2001). Isoelectric focusing nonporous silica reversed-phase high-performance liquid chromatog-raphy/electrospray ionization time-of-flight mass spectrometry a three-dimensional liquid-phase protein separation method as applied to the human erythroleukemia cell-line. Rapid Commun Mass Sp. 15(18), 1649-1661. 

Chloupek, R.C., Hancock, W.S., Marchylo, B.A., Kirkland, J.J., Boyes, B.E., Snyder, L.R. (1994). Temperature as a variable in reversed-phase high-performance liquid chromatographic separations of peptide and protein samples, n. Selectivity effects observed in the separation of several peptide and protein mixtures. J. Chromatogr. A 686, 45-59. 

Chong BE, Lubman DM, Miller FR, et al. Rapid screening of protein profiles of human breast cancer cell lines using non-porous reversed-phase high performance liquid chromatography separation with matrix-assisted laser desorption/ionization time-of-flight mass spectral analysis. Rapid Commun. Mass Spectrom. 1999 13 1808-1812. 

GRADIENT ELUTION FOR THE REVERSED-PHASE HIGH-PERFORMANCE LIQUID CHROMATOGRAPHIC SEPARATION OF COLOUR PIGMENTS IN THE EXTRACTS OF PAPRIKA (<CAPSICUM ANNUUM). ELUENT A, METHANOL-ACETONITRILE (80 20, V/V) ELUENT B, BIDISTILLED WATER... 

M. Weissenberg, I. Schaeffler, E. Menagem, M. Barzilai and A. Levy, Isocratic non-aqueous reversed-phase high-performance liquid chromatography separation of capsanthin and cap-sorubin in red peppers (Capsicum annuum L.), paprika and oleoresin. J. Chromatogr.A 757... 

Aboul-Enein HY, Al-Duraibi lA. 1998. Separation of several free polyamines and their acetylated derivatives by ion-pair reversed-phase high performance liquid chromatography. Biomed Chromatogr 12 291-293. 

Price, W.P., Jr., and Deming, S.N. (1979), Optimized Separation of Scopoletin and Umbelliferone and cis-trans Isomers of Ferulic and p-Coumaric Acids by Reverse-Phase High-Performance Liquid Chromatography, Anal. Chim. Acta, 108, 227-231. 

Reddy TV, Weichman BE, Lin EL, et al. 1993. Separation and quantitation of nitrobenzenes and their reduction products nitroanilines and phenylenediamines by reversed-phase high-performance liquid chromatography. J Chromatogr A655 331-335. 

Friedman, M., Levin, C. E. (1992). Reversed-phase high-performance liquid chromatographic separation of potato glycoalkaloids and hydrolysis products on acidic columns. J. Agric. Food Chem., 40, 2157-2163. 

In this unit, methods for reversed-phase high-performance liquid chromatography (HPLC) are described for the analysis of polyphenolics. HPLC analysis can be employed in an easy and fast manner to obtain an accurate elucidation and quantification of individual polyphenolic compounds found in plant-based materials. The separation of each polyphenolic is based on the polarity differences among polyphenolics with structural similarities and uses various combinations of mobile and stationary phases. 

JL Glajch, JJ Kirkland, J Kohler. Effect of column degradation on the reversed-phase high-performance liquid-chromatographic separation of peptides and proteins. J Chromatogr 384 81-90, 1987. 

MG Scanlon, PKW Ng, DE Lawless, W Bushuk. Suitability of reversed-phase high-performance liquid chromatographic separation of wheat proteins for long-term statistical assessment of breadmaking quality. Cereal Chem 67 395-399, 1990. 

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