Retinol-binding protein interaction with retinoids

The peculiar spectral properties of CRBP-bound retinol and RME are indicative of specific ligand-protein interactions (see Fig. 2) Instead, other holo-retinoid-binding proteins exhibit less characteristic spectra. For example the absorption spectrum of the complex of retinol with plasma retinol-binding protein (RBP) is characterized by a single, well-shaped peak centered at approx 328 nm On the other hand, the absorption spectra of some CRBP-bound retinoids, like CRBP-bound a l-trans retinal (12,13), do not resemble those of CRBP-bound retinol and RME. 

Malpeli, G, Stoppini, M., Zappom, M.C, Folk, C, and Berm, R (1995) Interactions with retinol and retinoids of bovine cellular retinol-binding protein. Eur. J Biochem, 229,486-493... 

Bemi R, Clerici M, Malpeli G, Cleris L, Formelli F (1993) Retinoids in vitro interaction with retinol-binding protein and influence on plasma retinol. FASEB Jl 179-1184... 

The cellular metabolism of retinoids involves the interaction of several enzymes together with a number of retinoid-binding proteins44 64 (Fig. 3). While these binding proteins have been well described in mammals, little is known about their properties in fish, although orthologues of the mammalian proteins do exist29,55. Retinol can be metabolized to retinal and, in turn, be irreversibly converted to RA in tissues that... 

Chen Y. Saari JC, Noy N. Interactions of all-frans-retinol and long-chain fatty acids with interphotoreceptor retinoid-binding protein. Biochemistry 1993 32 11,311-11,318. 

Retinoids (derivatives of retinol) act like steroid hormones and interact with specific receptor proteins in the cell nucleus. The ligand-receptor complexes bind to specific DNA sequences, where they control the transcription of particular genes. 

Herr F, Ong DE (1992) Differential interaction of lecith/n retinol acyltransfersase with cellular retinoid-binding proteins. Biochemistry 31 6748-6755... 

Eppinger TM, Buck J, Hammerling U (1993) Growth control or terminal differentiation endogenous production and differential activities of vitamin A metabolites in HL-60 cqWs. J Exp Med 178 1995-2005 Herr F, Ong DE (1992) Differential interaction of lecithin-retinol acyltransferase with cellular retinoid-binding proteins. Biochemistry 31 6748-6755... 

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