Resveratrol metabolites

Urpi-Sarda M, Jauregui O, Lamuela-Raventos RM, Jaeger W, Miksits M, Covas MI and Andres-Lacueva C. 2005. Uptake of diet resveratrol into the human low-density lipoprotein. Identification and quantification of resveratrol metabolites by liquid chromatography coupled with tandem mass spectrometry. Anal Chem 77(10) 3149—3155. 

Zamora-Ros et al [2006] carried out the first work that assessed the bioavailability of resveratrol (provided by different wines) in a regular intervention during 28 day. The analyses were performed by LC-MS/MS. In the first study, 10 healthy males were recruited to consume 300 mL/day of sparkling wine (1.19 mg resveratrol/1). After 28 day of supplementation, urinary trans-and m-resveratrol-3-O-glucuronides were 75 and 38 nmol/g creatinine, respectively. In the second study, 10 healthy females were selected to consume 200 mL of white wine (1.99 mg resveratrol/L) or 200 mL of red wine (12.8 mg resveratrol/L) in a crossover clinical trial. Likewise after 28 days only resveratrol metabolites were detected in morning urine, trans- (205 and 473 nmol/g creatinine) and ra-resveratrol-3-O-glucuronidcs (58 and 140 nmol/g creatinine) were found after white and red wine intake, respectively. Those studies showed that urinary excretion was dose dependent. Furthermore, slight amounts of resveratrol metabolites were also detected at baseline periods. No free resveratrol or piceid were detected in any of the studies. 

Urinary excretion mainly took place in the first 4 h after consumption (77% of total excretion), although resveratrol metabolites remained in urine between 12and24 h after intake. Free resveratrol, 2 glucuronides, and the 3-sulfate excreted in the urine 24 h after intake were below 0.04, 2, 9, and 11 % of the 0.5 mg provided, respectively. At higher dose (5 mg) resveratrol, glucuronides and sulfate recoveries in the urine at 24 h were 0.1, 0.5, 3, and 5% of the dose, respectively. In urinary excretion, the sulfate forms were also higher than the glucuronide and free forms. 

Burkon A, Somoza V. 2008. Quantification of free and protein-bound iraw-s-resveratrol metabolites and identification of traws-resveratrol-C/O-conjugated diglucuronides— Two novel resveratrol metabolites in human plasma. Mol Nutr Food Res 52 549-557. 

Zamora-Ros R, Urpi-Sarda M, Lamuela-Raventos RM, Estruch R, Vazquez-Agell M, Serrano-Martinez M, Jaeger W, Andres-Lacueva C. 2006. Diagnostic performance of urinary resveratrol metabolites as a biomarker of moderate wine consumption. Clin Chem 52 1373-1380. 

All these investigations support the use of concentrations of urinary resveratrol metabolites as a useful nutritional biomarker of wine consumption in clinical and epidemiological studies. This biomarker would provide an additional and more accurate tool to evaluate the relationship between wine intake and health etfects. 

Zamora-Ros, R., Urpi-Sarda, M., Lamuela-Ravent6s, R.M. et al. (2009). Resveratrol metabolites in urine as a biomarker of wine intake in fiee-Uving subjects the PREDIMED Study. Free Radical Biology Medicine, 46,1562-1566. 

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