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The Rieske protein II (SoxF) from Sulfolobus acidocaldarius, which is part, not of a bci or b f complex, but of the SoxM oxidase complex 18), could be expressed in E. coli, both in a full-length form containing the membrane anchor and in truncated water-soluble forms 111). In contrast to the results reported for the Rieske protein from Rhodobacter sphaeroides, the Rieske cluster was more efficiently inserted into the truncated soluble forms of the protein. Incorporation of the cluster was increased threefold when the E. coli cells were subject to a heat shock (42°C for 30 min) before induction of the expression of the Rieske protein, indicating that chaperonins facilitate the correct folding of the soluble form of SoxF. The iron content of the purified soluble SoxF variant was calculated as 1.5 mol Fe/mol protein the cluster showed g values very close to those observed in the SoxM complex and a redox potential of E° = +375 mV 111). [Pg.146]

The dynamics of inter- vs intrastrand hole transport has also been the subject of several theoretical investigations. Bixon and Jortner [38] initially estimated a penalty factor of ca. 1/30 for interstrand vs intrastrand G to G hole transport via a single intervening A T base pair, based on the matrix elements computed by Voityuk et al. [56]. A more recent analysis by Jortner et al. [50] of strand cleavage results reported by Barton et al. [45] led to the proposal that the penalty factor depends on strand polarity, with a factor of 1/3 found for a 5 -GAC(G) sequence and 1/40 for a 3 -GAC(G) sequence (interstrand hole acceptor in parentheses). The origin of this penalty is the reduced electronic coupling between bases in complementary strands. [Pg.70]

The comparison of coronal and photospheric abundances in cool stars is a very important tool in the interpretation of the physics of the corona. Active stars show a very different pattern to that followed by low activity stars such as the Sun, being the First Ionization Potential (FIP) the main variable used to classify the elements. The overall solar corona shows the so-called FIP effect the elements with low FIP (<10 eV, like Ca, N, Mg, Fe or Si), are enhanced by a factor of 4, while elements with higher FIP (S, C, O, N, Ar, Ne) remain at photospheric levels. The physics that yields to this pattern is still a subject of debate. In the case of the active stars (see [2] for a review), the initial results seemed to point towards an opposite trend, the so called Inverse FIP effect , or the MAD effect (for Metal Abundance Depletion). In this case, the elements with low FIP have a substantial depletion when compared to the solar photosphere, while elements with high FIP have same levels (the ratio of Ne and Fe lines of similar temperature of formation in an X-ray spectrum shows very clearly this effect). However, most of the results reported to date lack from their respective photospheric counterparts, raising doubts on how real is the MAD effect. [Pg.78]

When subjects consumed 7 oz. of 80 proof alcohol (in orange juice) in 20 minutes, mean blood levels rose only to 0.07% (Fig. 107). This finding was in line with results reported by Sidell and Pless (1971). Only 2 subjects exceeded a level of. 10%. The rate of elimination was linear after 2 hours, averaging 15 mg% hourly, somewhat less than reported in many other studies. The mean peak value did not exceed the (then) legal limit of 0.10, even after consuming what most would consider a large dose of alcohol. Similar results have been reported by other investigators. [Pg.337]

An example of the possibility that network scission experiments may be subject to topological interpretation is suggested by the results reported by Hookway and Shelton (2). Of particular interest is the degelation point where the network dissolves. (Degelation implies transition through a gel point that may or may not be related structurally to the usual non-gel to gel transition observed in the corresponding network synthesis. The data (ref. 2, Fig. 3) show that hydrogen peroxide causes the release of about 0.5 mole... [Pg.356]

How, specifically, does an analytical chemistry laboratory assure the quality of its work The purpose of this monograph is to discuss the processes utilized by analytical chemistry laboratories through which the results reported to their customers and clients, whether internal to their company or external, are assured to be of the highest quality and greatest accuracy possible. The methods, procedures, and techniques employed by these laboratories for the individual analyses that they perform are what are called into question and tested. In most cases, methods of statistics must be applied because the measurement techniques are subject to errors that often cannot be identified or compensated. [Pg.11]

In view of those experiments some results reported by later authors who did not find NOz among the thermal decomposition products of nitrocellulose should be subjected to revision. There is in fact, a serious controversy concerning this question as shown in the following paragraphs. [Pg.309]

Large-pore titanosilicates developed after TS-1, for example, Ti-beta, Ti-ITQ-7, Ti-MCM-41 and Ti-MCM-48, have been claimed to have advantages for the oxidation of bulky alkenes because of their pore size [15-17, 66, 67]. However, none of them is intrinsically more active than TS-1 in the reactions of small substrates that have no obvious diffusion problem for the medium pores. Therefore, in parallel with developing large pore titanosilicates, the search for more intrinsically active ones than TS-1 is also an important research subject. The catalytic performance of Ti-MWW is compared in the oxidation of 1-hexene with H202 with that of TS-1 and Ti-beta. Consistent with the results reported elsewhere [15-17], TS-1 showed higher conversion than Ti-beta with a similar Ti content. However, Ti-MWW exhibited activity about three times as high as TS-1 based on the specific conversion per Ti site (TON). [Pg.139]

Sponsors and monitors responsibilities in complying with GCPs are also subject to serious repercussions under 21CFR 312.58. FDA inspectors are allowed to examine sponsors files and the interventions of monitors site visits to assure that GCP compliance was executed. Case report forms and clinical results are subjected to the same scrutiny that are applied to the investigators responsibilities. If during an FDA inspection discrepancies are found in any form among the investigator, sponsor, and, when appropriate, the... [Pg.1929]


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