Results Obtained with Calf Thymus DNA

7 Temperature Effect on Fluorescence of the Ethidium Bromide-DNA Complex 

The melting temperature, Tm, of DNA is equal to that when the two macromolecule strands split. If we consider that upon complete denaturation, the fluorescence intensity is 0.6, Tm can be calculated at half denaturation at 0.8 and so is 70.5 0.5°C. This value is equal to that found when OD is measured as a function of temperature. One 

Duhamel, J., Kanyo, J., Dinter-Gottlieb, G. and Lu, P. (1996). Fluorescence emission of ethidium bromide intercalated in defined DNA duplexes Evaluation of hydrodynamics components. Biochemistry, 35, 16687-16697. 

Chaires, J.B. and Waring, M.J. (2003). Energetics of echinomycin binding to DNA. Nucleic Acids Research, 31,6191-6197. 

Marmur, J. and Doty, P. (1959). Heterogeneity in deoxyribonucleic acids. I. Dependence on composition of the configurational stability of deoxyribonucleic acids. Nature, 183, 1427-1429. 

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