Intercalation Ethidium bromide

Among these methods, hydrodynamic measurements of sedimentation coefficients and viscosity are classical [43,47]. The insertion of a true intercalator (ethidium bromide for example) between the stacking of bases produces... 

Fluorescence-based indicator reactions can also be coupled to measurements of enzymatic activity. For example, very sensitive assays of endonuclease activity can be performed using as a substrate complexes of double stranded DNA containing intercalated ethidium bromide, which is strongly fluorescent. On cleaving or nicking the DNA, the intercalated ethidium is released into the solution causing a pronounced decrease in fluorescence intensity. 

Fig. 2. Degradation kinetic of lipoplexes obtained with stable lipid 3 and pH labile lipids 2 and 4 at pH 5, and charge ratios 1.7 and 6. DNA release induced by degradation is measured by the % of fluorescence of intercalated ethidium bromide at 590 nm " 260 nm (100% refers to naked DNA)...

RADACK may also account for the experimentally observed reduced probability of getting a damage at the site of binding of a minor groove ligand that has a preference for a specific sequence (a natural polyamine [16] ora therapeutic radioprotector, Ethyol [17]) as it does for the experimentally observed effect of an intercalator (ethidium bromide) [18]. 

Straight SOmer and A5-bulge bent SOmer/ intercalated ethidium bromide Series of oligos... 

G-quadruplex DNA can convert to the duplex form in the presence of its complementary strand [82]. Such a conformational change can also be detected using P4 [83]. The overall strategy is illustrated in Scheme 1.18. The intercalator ethidium bromide (EB) is introduced into the assay solution. Electrostatic interactions between G-quadruplex-Fl and P4 keep them... 

It has been shown that DNA intercalators (ethidium bromide and proflavine) form complexes with single-stranded DNA and homopolynucleotides [3]. It was of interest, therefore, to determine the effect of intercalators on the degradation of the homopolymer (ADP-ribose)jj by the poly (ADP-ribose) glycohydrolase. 

TABLE 2. Derived rate constants for forward electron transfer processes observed with intercalated ethidium bromide (EB ) or acridine orange (AO) at implied distances (R). 

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