Resonance function energy transfer

The analysis of carotenoid identity, conformation, and binding in vivo should allow further progress to be made in understanding of the functions of these pigments in the photosynthetic machinery. One of the obvious steps toward improvement could be the use of continuously tuneable laser systems in order to obtain more detailed resonance Raman excitation profiles (Sashima et al 2000). This technique will be suitable for the investigation of in vivo systems with more complex carotenoid composition. In addition, this method may be applied for the determination of the energy of forbidden Sj or 2 Ag transition. This is an important parameter, since it allows an assessment of the energy transfer relationship between the carotenoids and chlorophylls within the antenna complex. 

Jovin, T. and Arndt-Jovin, D. (1989). FRET microscopy Digital imaging of fluorescence resonance energy transfer. Application in cell biology. In Cell Structure and Function by Microspectrofluorometry (Kohen, E., JG, H. and Ploem, J., eds.). Academic Press, London, pp. 99-117. 

Resonance energy transfer is thus a powerful tool for understanding the functions of glycoproteins. 

Resonance energy transfer between the aromatic amino acids proceeds by very weak coupling between the donor and acceptor.151,52) Very weak coupling implies that the interaction between the donor and acceptor wave functions is small enough so as not to perturb measurably the individual molecular spectra. This transfer process, which is distinct from the trivial process of absorption of an emitted photon, involves radiationless deexcitation of an excited-state donor molecule with concomitant excitation of a ground-... 

As a consequence of the quantum-mechanical selection rules for resonance energy transfer involving the spin wave functions of the donor and acceptor,... 

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