Resin dialysis technique

A number of alternative approaches are available as technical solutions for the above-mentioned problems (e.g., Hamer, 1982 Lilly, 1982 Meiorella et al., 1981) vacuum membrane retractive and extractive fermentation, and biphasic processing (Mattiasson, 1983 Reisinger et al., 1987) and ion-exchange resin culture techniques. Experimental tests of dialysis culture processes are described in the literature for ammonium-lactate fermentation of whey (Stieber et al., 1977), and an improved mathematical model was later developed (Stieber and Gerhardt, 1979) incorporating P inhibition. Recently,... 

After preparation, colloidal suspensions usually need to undergo purification procedures before detailed studies can be carried out. A common technique for charged particles (typically in aqueous suspension) is dialysis, to deal witli ionic impurities and small solutes. More extensive deionization can be achieved using ion exchange resins. 

Brunswick (1971) had only achieved 20% incorporation of label previously in their respective studies on yeast alcohol dehydrogenase, Brunswick and Cooperman (1973) achieved more than 70 % incorporation of ligand into phosphofructokinase using either the dialysis or resin technique with the photoaffinity label of cyclic AMP. 

Since so many of the applications of monodisperse particles are in biochemical fields, it is a fortunate circumstance that most of the syntheses may be carried out with little or no surfactant or other additives. While the early work was usually carried out with conventional surfactants, today many latices with uniform particle size polymers are simply initiated with an inorganic persulfate. Buffers and some other water-soluble electrolytes may be added mainly to modify the particle size distribution. The few water-soluble components that may be present are subsequently removed by such techniques as ion exchange using a mixed bed of ion-exchange resins, centrifugation, dialysis, or water-replacement using suitable membranes or hollow fiber equipment. 

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