Research on Cypridina Luminescence before

During the period between 1916 and the early 1950s, Harvey, his associates and students made great efforts to study various aspects of the Cypridina bioluminescence, which was described by Harvey in his book Bioluminescence (1952). It should be pointed out here 

The extracted crude luciferin was purified by Anderson s benzoy-lation method mentioned above (Anderson, 1935), followed by partition chromatography on a cellulose powder column using a mixture of ethyl acetate-ethanol-water (5 2 3 Tsuji, 1955) as the solvent, again in a hydrogen atmosphere. The luciferin obtained (3 mg) was highly pure by paper chromatography, and appeared suitable for crystallization. I 

Improved purification method of Cypridina luciferin. The purification of Cypridina luciferin became remarkably simple and easy after some of the properties of this substance were known. The following method was used to obtain the large quantity of luciferin needed for the study of its chemical structure. The method consists of three steps and takes less than eight hours to obtain crystallized luciferin. 

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