Reporter markers Green fluorescent protein

As human skin fibroblasts are recalcitrant to transfection, we wished to include an easily traceable marker, which can either be used as an in-frame fusion protein, or as a separately expressed protein. The widely applicable green fluorescent protein (GFP) reporter system, originally isolated from the jellyfish Aequorea victoria, and adapted for efficient expression in human cells and for, elevated levels of fluorescence, provides these properties. The GFP system has been used for example, to study protein trafficking to the mitochondrial matrix and the mitochondrial outer membrane. Therefore, by using confocal laser scanning imaging we may get an impression of the intracellular locahzation of a CPTl-GFP fusion protein after transient or stable transfection. 

Using a vector carrying a splice acceptor sequence placed upstream of a reporter gene (such as lacZ or GFP [green fluorescent protein]), different types of regulatory or gene sequences can be trapped (31-33). ES cell-chimeric embryos are stained for the histochemical marker to reveal expression domains of the tr jped elements (31-33). On the basis of the expression pattern information... 

Yeh E., Gustafson K., and Bouhanne G.L. 1995. Green fluorescent protein as a vital marker and reporter of gene expression in Drosophila. Proc. Natl. Acad. Sci. 92 7036—7040. 

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