Reliable chromatographic performance

The trend has been toward the increased nse of GC in the petrolenm industry. This is due to the relatively low cost of a gas chromatographic system that can provide more detailed analyses as well as the detection of trace components. Gas chromatography has also provided a means for online process monitoring. Future utilization of GC will involve the establishment of basic relationships between composition and performance parameters. This will allow the substitution of this rapid and reliable chromatographic analysis for the empirical methods from the past. 

It has been argued that in a typical 2DLC proteomic experiment, with only a limited number of fractions submitted for analysis in the second LC dimension, chromatographic peak capacity is less than 1000. This value is considerably lower than the expected sample complexity. Additional resolution is offered by MS, which represents another separation dimension. With the peak capacity defined as the number of MS/MS scans (peptide identifications) accomplished within the LC analysis time, the MS-derived peak capacity was estimated to be in an order of tens of thousands. While the MS peak capacity is virtually independent of LC separation performance, the complexity of the sample entering the MS instrument still defines the quality of MS/MS data acquisition. The primary goal of 2DLC separation is to reduce the complexity of the sample (and concentrate it, if possible) to a level acceptable for MS/MS analysis. What is the acceptable level of complexity to maintain the reliability and the repeatability of DDA experiments remains to be seen. 

Sidhu et al [86] developed a reliable and simple high performance liquid chromatographic method for the routine analysis of pharmaceutical dosage forms using a Cig Bondapak reversed-phase column with a binary solvent system consisting of... 

An overview of HPLC instrumentation, operating principles, and recent advances or trends that are pertinent to pharmaceutical analysis is provided in Chapter 3 for the novice and the more experienced analyst. Modern liquid chromatographs have excellent performance and reliability because of the decades of refinements driven by technical advances and competition between manufacturers in a two billion-dollar-plus equipment market. References to HPLC textbooks, reference books, review articles, and training software have been provided in this chapter. Rather than summarizing the current literature, the goal is to provide the reader with a concise overview of HPLC instrumentation, operating principles, and recent advances or trends that lead to better analytical performance. Two often-neglected system parameters—dwell volume and instrumental bandwidth—are discussed in more detail because of their impact on fast LC and small-bore LC applications. 

J van Schoonhoven, J Schrijver, H van den Berg, GRMM Haenen. Reliable and sensitive high-performance liquid chromatographic method with fluorometric detection for the analysis of vitamin B-6 in foods and feeds. J Agric Food Chem 42 1475-1480, 1994. 

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