Recombination of DNA

Marillonnet, S., Giritch, A., Gils, M., Kandzia, R., Klimyuk, V, and Gleba, Y. (2003). In plant engineering of viral RNA replicons efhcient assembly by recombination of DNA modules delivered by Agrobacterium. Proc. Natl. Acad. Sci. U.S.A. 101(18) 6852-6857. 

Stanley Cohen and Herbert Boyer Stanford, CA, USA method of recombination of DNA developed... 

A library of parent DNA sequences encoding for the desired protein is chosen. Sequence diversity is created or increased through a mutagenesis step, either by introduction of random point mutations through error-prone PCR or by recombination of DNA fragments such as DNA shuffling or RACHITT. 

Current molecular biology and biotechnology are based on two key processes (i) site-selective scission of DNA by restriction enzymes and (ii) recombination of DNA fragments by another enzyme, known as ligase. These methods, first reported in the 1970s, are almost perfect as long as we manipulate small DNA such as the plasmid DNA of bacteria and genome DNA of virus, which are composed of several thousand... 

Figure 5.36. Exon Shuffling. Exons can be readily shuffled by recombination of DNA to expand the genetic repertoire.

DNA polymerases are ubiquitous enzymes required for replication, repair and recombination of DNA genomes [121], Most DNA polymerases are multi-functional enzymes which exhibit, in addition to the polymerase activity, a 3 to 5 exonuclease activity involved in the fidelity of DNA synthesis (proof-reading). 

Marillonnet, S., et al., In planta engineering of viral RNA replicons efficient assembly by recombination of DNA modules delivered by Agrobacterium. Proc Natl Acad Sci USA,... 

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