Identification of Bacterial Colonies that Contain Recombinant DNA

2 Identification of Bacterial Colonies that Contain Recombinant DNA 

There are several methods to identify bacterial colonies harboring recombinant plasmid. One of the more commonly used methods is referred to as a-complementa-tion. The pUC series of plasmids contains the coding sequence for the first 146 amino acids of the amino terminus of the (S-galactosidase gene (lacZ). Within this 

EicoRI Sacl Kpnt Smal Bam HI Xbal Safi Pstl Sphl Hindlll 

Transform into E.coli and select with ampicillin  

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