Application of recombinant DNA

Microbes for Industiial and Agiicultural Applications, Dekker, NY, 1993. Click, B. R. and J. J. Pasternak, Molecular Biotechnology Trinciples and Applications of Recombinant DNA, ASM Press, Herndon, VA, 1994. Bajpai, Rakesb K., and Ales Prokop, eds. Recombinant DNA Technology II, Annals of the New York Academy of Sciences, vol. 721, 1993. 

Experimental approaches that have afforded major insights to the processes described in this chapter include (1) use of yeast mutants (2) application of recombinant DNA techniques (eg, mutating or eliminating particular sequences in proteins, or fusing new sequences onto them and (3) development of in vitro... 

The molecular causes of a number of heritable diseases of bone (eg, osteogenesis imperfecta) and of cartilage (eg, the chondrodystrophies) are being revealed by the application of recombinant DNA technology. 

The application of recombinant DNA technology is revolutionizing the field of hematology. 

Glick B.R Pasternak J.J. (1994) Molecular Biotechnology Principles and Applications of Recombinant DNA. Washington, D.C. American Society for Microbiology. 

Downham, M., Busby, S., Jefferis, R., and Lyddiatt, A., Immunoaffinity chromatography in biorecovery an application of recombinant DNA technology to generic adsorption processes, /. Chromatogr., 584, 59, 1992. 

Examples of the early application of recombinant DNA technology in medicine are the development of recombinant human growth hormone human insulin human interferons, thought to have anticancer activity in addition to antiviral activity interleukins (regulatory proteins from lymphocytes that are believed to be important in the treatment of immunodeficiency diseases and cancer) tumor necrosis factor epidermal and bone marrow progenitor cell growth factors and the production of vaccines (Table 12.1). 

Click BR, Pasternak JJ, In Molecular Biotechnology Principles And Applications of Recombinant DNA (Eds Click BR, Pasternak JJ), pp. 145-169. American Society for Microbiology, Washington DC, 1998. 

One of the major applications of recombinant DNA technology has been to produce large amounts of commercially relevant proteins, including enzymes, receptors, and peptide messengers of various sorts. The sequences of these proteins, at least in the initial stages of investigation and production, have been those found in nature, so that the structure and function of the protein products of cloning would be the same as those of natural proteins extracted from tissue, serum, and so forth. 

Application of recombinant DNA technology to clone and express the gene for bovine somatotropin has made it possible to obtain an unlimited amount of the hormone and provides further an opportunity to investigate benefits that may be derived from controlling milk production through supplemental administration of bovine somatotropin on a commercial scale. Central to studies conducted to investigate the safety and efficacy of recombinant-derived bovine somatotropin is the need for a sensitive analytical method that could be used to estimate bovine somatotropin levels in various biological fluids. 

Unterman, R., Mondello, F. J., Brennan, M. J., Brooks, R. E., Mobley, D. P., McDermott, J.B. Schwartz, C. C. (1987b). Bacterial treatment of PCB contaminated soils Prospects for the application of recombinant DNA technology. In Proceedings of the Second International Conference on New Frontiers for Hazardous Waste Management, pp. 259-64. U.S. Environmental Protection Agency (EPA6/600/9-87/018F). 

Highlights attending receptor pharmacogenetics research are presented chronologically in Table 3. It is evident from the record that the advent and widespread application of recombinant DNA technology has greatly... 

Glick BR, Pasternak JJ (1994) Molecular biotechnology principles and applications of recombinant DNA. ASM Press, Washington, DC... 

Be aware of specific applications of recombinant DNA technology, such as the production of useful proteins, the construction of useful organisms, uses in medical diagnosis, for gene therapy, and for basic experimental studies. 

The application of recombinant DNA techniques to the study of neuropeptide biosynthesis is essential because these techniques facilitate structural analyses and evolutionary studies, clarify biosynthetic pathways, provide the necessary background and probes for analysis of synthesis regulation at the levels of mRNA and gene transcription and assist in classical genetic experimentation. Examples of these benefits are cited and problems encountered in insect systems are discussed. 

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