Recombinant microorganisms, optimization

The use of recombinant microorganisms for cofermentation is one of the most promising approaches in the field of bioethanol production, though their use for large-scale industrial processes still requires fine-tuning of the reliability of the entire process (2). The technical hurdles of cofermentation increase when real biomass hydrolysates have to be fermented. In fact, whatever the biomass pretreatment, the formation of degradation byproducts that could inhibit the fermentation usually requires the addition of a further detoxification step. Therefore, the production of ethanol from hydrolysates should be considered in its entirety, from the optimal pretreatment to the choice of the proper fermentation process. 

Catalyst optimization, application scope, reaction parameters, case studies Microorganism screening, recombinant biocatalyst, reaction parameters, product isolation... 

Recombinant DNA techniques. Mutagenesis and selection techniques based on classical bacteriological and genetic methods are common procedures to optimize and standardize microorganisms used in food fermentations. Recombinant DNA techniques offer the potential of altering the properties of microorganisms more precisely in terms of production efficiency, product quality, safety, and diversity... 

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