Preparative capillary

S. Blomherg and J. Roeraade, Preparative capillary gas cliromatography. IF Fraction collection on rtaps coated with a very tliick-film of immobilized stationary phase , J. Chromatogr. 394 443-453 (1987). 

J. P. E. M. Rijks and J. A. Rijks, Programmed cold sample intr oduction and multidimensional preparative capillary gas cliromatogr aphy. Part I introduction, design and operation of a new mass flow contr olled multidimensional GC system , J. High Resolut. Chromatogr. 13 261 -266 (1990). 

Chemical surface modifications The first surface modification for the purpose of eliminating EOF and protein adsorption was recommended by Hjerten.28 The attachment of vinyl silanes allowed the polymerization of a variety of molecules to the surface. Most of the chemical modifications used for preparing capillaries for electrophoresis originated from the experience acquired over the years preparing GC and LC stationary phases. Chemical modification should conform to certain requirements, including the prevention of adsorption, the provision of stable and constant EOF over a wide pH range, chemical stability, ease of preparation, and reproduciblity of preparation. The effects of silanization of the inner surface of capillaries on electrophoretic separations have been extensively studied.26-29... 

Another approach is similar to that used in for the preparation of polymer-layer open tubular GC columns (PLOT). Horvath s group prepared capillaries with a porous polymer layer as shown in Fig. 13 by in situ polymerization of vinylbenzylchloride and divinylbenzene [183]. The reaction of the N,N-di-methyldodecylamine with chloromethyl groups at the surface simultaneously afforded strong positively charged quaternary ammonium functionalities and attachment of C12 alkyl chains to the surface. The unreacted chloromethyl groups... 

Eglinton et al. [688] described a practical approach for isolation of individual compounds from complex organic matrices for natural abundance radiocarbon measurement. This approach uses an automated preparative capillary gas chromatography (PCGC) to separate and recover sufficient quantities of... 

Thus, it can be said that the capillary itself is the most important CE equipment consideration when considering CE performance. Considerations must be given to capillary ID, OD, and length. Other considerations include the cleanliness of the window and the cutting of the capillary ends. A poorly selected or prepared capillary will cause poor results and much valuable time lost. 

Identification of these components was based on GC/MS and retention index information. Novel compounds were isolated by preparative capillary gas chromatography and spectroscopically identified by interpretation of infrared, nuclear magnetic resonance and mass spectra. In... 

Eriksson, K-O., Palm, A., and Hjerten, S. (1992). Preparative capillary electrophoresis based on adsorption of the solutes (proteins) onto a moving blotting membrane as they migrate out of the capillary. Anal. Biochem. 201, 211-215. 

Deforce, D.L.D., Kokotos, G., Esmans, E.L., De Leenheer, A.P., Van den Eeckhout, E.G. Preparative capillary zone electrophoresis in combination with off-line graphite furnace atomic absorption for the analysis of DNA complexes formed by a new aminocoumarine platinum(II) compound. Electrophoresis 19, 2454-2458 (1998)... 

Name Propylene glycol Sample preparation Capillary film, sodium chloride cell... 

---