Recombinant human bone morphogenetic rhBMP

Figure 4.4 Design and fabrication of the osteophilic multilayer architecture. Components of the film (a) Chitosan (75-85% deacetylated chitin) a polycation (b) Poly(acrylic acid) (PAA) (c) A hydrolytically degradable poly(p-amino ester) (Poly2), a polycation and (d) osteoinductive recombinant human bone morphogenetic protein 2 (rhBMP-2) (e) Schematic of the modular electrostatic assembly. Osteoconductive hydroxyapatite (HAP) is complexed with chitosan and incorporated into nanoscale thick films along with poly(acrylic acid) in a bilayer architecture. A hydrolytically degradable poly(P-amino ester)-based multilayer fihn incorporating osteoinductive rhBMP-2 lays atop the osteoconductive layer, (f)...

Polyurethane scaffolds Recombinant human bone morphogenetic protein (rhBMP)... 

In addition, the concept of osteostimulation by growth-supporting cytokines such as transforming growth factor- 3 (TGF-1 ), insuUn-Uke growth factor-1 (IGF-1), tumor necrosis factor-a (TNF-a) or recombinant human bone morphogenetic proteins (rhBMPs Urist, 1965) continues to be the subject of extensive clinical research, and has indeed also been applied therapeutically (Marks and Popoff, 1988 Service, 2000). 

Recently, a novel macroporous (surface area up to 1.5m cm ), zirconia-based cell carrier biomaterial (Sponceram Figure 10.5), which may be either partially or fully stabilized with Ca, Mg or Y, has been developed that can easily be coated not only with osteoconductive hydroxyapatite but also with the osteoinductive recombinant human bone morphogenetic protein, rhBMP-2. The pore size of this material is about 600 xm, and the porosity may be up to 85% (Roker et al., 2009). Subsequent cell proliferation studies performed in a bioreactor with preosteoblas-tic MC3T3-E1 cells showed that alkaline phosphatase (AP) activity attained a maximum level at day 5 in the presence of rhBMP-2. Likewise, a significant calcification of the extracellular matrix (ECM) occurred in the presence of rhBMP-2,... 

CoUagenous matrix (atelocoUagen) [72] Recombinant human bone morphogenetic protein-2 (rhBMP-2) FUm Bone graft substitute for bone reconstruction... 

Yasko, A.W. et al, The healing of segmental bone defects, induced by recombinant human bone morphogenetic protein (rhBMP-2). A radiographic, histological, and biomechanical study in rats, /. Bone Joint Surg. Am. 74, 659,1992. 

Porteg T. J., Rathore, S., Ronse, J. C. and Denton, M. Biomolecules in tissue engineered medical prodncts (TEMPs) A case stndy of recombinant human bone morphogenetic protein-2 (rhBMP-2). Tissue Eng. Med. Prod. 1 150-171, 2004. 

Musculoskeletal A rare case of extraspinal ectopic bone growth has been reported after the use of rhBMP-2 (Infuse Bone Graft), a recombinant human version of bone morphogenetic protein 2 f5" ]. 

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