Recombinant gene technology

In the search for new vaccines against anthrax, manufacturers have aimed for better quality purified protective antigen vaccines with better adjuvants, vaccines made through recombinant gene technology, and mutant vaccines with altered protective antigens. 

Another gene encoding Saccharomyces cerevisiae has recently been characterized [26]. It may well be that in the future, using recombinant gene technology, more alternatives to E. coli or Erwinia 1-ASP will become available. 

Recombinant DNA technology can also be used to design genes that encode for proteins with desired features [34]. The gene can be incorporated into a plasmid, which is then used to transform a bacterial host such as Escherichia coli. Finally, the production of the desired amino acid polymer is performed by the host with a precisely defined sequence and near absolute monodispersity [29, 35]. 

Recombinant DNA technology can also be used to create modified proteins that are readily purified by affinity chromatography. The gene of interest is Unked to an oligonucleotide sequence that encodes a carboxyl or amino terminal extension to the encoded protein. The... 

Sickle cell disease again provides an excellent example of how recombinant DNA technology can be applied to the smdy of human disease. The substitution of T for A in the template strand of DNA in the P-globin gene changes the sequence in the region that corresponds to the sixth codon from... 

Section IV describes the structures and functions of the nucleotides and nucleic acids, and covers many major topics such as DNA replication and repair, RNA synthesis and modification, and protein synthesis. It also discusses new findings on how genes are regulated and presents the principles of recombinant DNA technology. 

The advantages of recombinant DNA technology are enormous, as the following example shows. Somatostatin is a hormone that inhibits the secretion of pituitary growth hormone. The researchers who first isolated somatostatin required nearly half a million sheep brairrs to produce 5 mg of the substance. Using a chemically synthesized gene, 9... 

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