Real-time polymerase chain reaction methods

Beller H. R., Kane S. R., Legler T. C., and Alvarez P. J. J. (2002) A real-time polymerase chain reaction method for monitoring anaerobic, hydrocarbon-degrading bacteria based on a catabolic gene. Environ. Sci. Technol. 36(18), 3977-3984. 

Real-Time Polymerase Chain Reaction Methods... 

Brezna B, Kuchta T (2008). A novel real-time polymerase chain reaction method for the detection of pecan nuts in food. Fur. Food Res. TechnoL, 226(5) 1113-1118. 

Brzezinski JL (2006). Detection of cashew nut DNA in spiked baked goods using a real-time polymerase chain reaction method. J. AOAC Int., 89(4) 1035-1038. 

Badosa E, Trias R, Pares D, Pla M and Montesinos E. 2008. Microbiological quality of fresh fruit and vegetable products in Catalonia (Spain) using normalised plate-counting methods and real time polymerase chain reaction (QPCR). J Sci Food Agric 88(4) 605-611. 

Pryor RJ, Wittwer GT. Real-time polymerase chain reaction and melting curve analysis. Methods Mol Biol. 2006 336 19-32. 

Lauhe I, Spiegelherg A, Butschke A, et al. (2003). Methods for the detection of beef and pork in foods using real-time polymerase chain reaction. Int. J. Food Sci. TechnoL, 38 111-118. 

Zhang X, Caggana M, Cutler TL, Ding X (2004) Development of a real-time polymerase chain reaction-based method for the measurement of relative allelic expression and identification of CYP2A13 alleles with decreased expression in human lung. J Pharmacol Exp Ther 311 373-381... 

Rark, H. G. Choi, J. J. Kim, H. S. Jung, S. U. Method and kit for detecting IDHl and IDH2 mutations using peptide nucleic acid (RNA)-based real-time polymerase chain reaction (RCR) clamping. 

Quantitative polymerase chain reaction, also called real-time RT-PCR or QPCR, is a method which employs insertion of a signal, such as fluorescence or enzyme activity, into PCR products generated by RT-PCR to determine the amount of messenger RNA (mRNA) in a tissue accurately. 

The TRAP (telomeric repeat amplification protocol) assay is a widely used method for detection of telomerase activity. This technique measures the telomerase activity present in cell extracts. Briefly, cellular extract containing telomerase activity is incubated with a telomeric substrate (a short strand of DNA onto which the telomerase wiU. attach the telomeric repeats) followed by polymerase chain reaction (PCR) amplification of the elongated telomere. Detection of the PCR product is by a number of methods, including gel electrophoresis, radiometric detection, ELISA, and real-time PCR detection. ... 

A DNA molecule can be amplified by the polymerase chain reaction (PCR) (section 6.2), if part of its sequence is known. One DNA molecule is sufficient to generate millions of identical copies in a controlled amplification reaction. With real-time PCR, the DNA quantity can be measured during the amplification reaction (section 6.2.4). Other methods of DNA quantification include DNA arrays (section 5.3) and, if available, biosensors (section 5.2). 

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