Reagents, in thin-layer chromatography

Modification of the Activity of Alumina by Addition of Water Stationary and Mobile Phases Typical Stationary and Mobile Phase Systems Used in the Separation of Various Inorganic Ions Spray Reagents in Thin-Layer Chromatography Protocol for Reagent Preparation... 

Krebs, K.G., Heusser, D., and Wimmer, H., Spray reagents, in Thin Layer Chromatography, A Laboratory Handbook, Stahl, E., Ed., Springer-Verlag, New York, 1969, p. 854. 

Spray Reagents in Thin-Layer Chromatography (continued)... 

The following section gives a summary for the preparation of the major spray reagents listed in the previous section (Spray Reagents in Thin-Layer Chromatography). Refer to the original literature for any reagents not listed here.1 4... 

It is often possible to increase the selectivity of detection by carrying out a sequence of reactions on one and the same chromatographic plate - a technique that is only possible in thin-layer chromatography. In principle it is possible to distinguish between two sorts of reagent sequence ( TYPE A and TYPE B ), which are discussed in this and the next volume. 

In addition to the reagent sequences with clearly detectable reaction mechanism, whict have already been described, many sequences of reagents not covered by any of th< reaction types described have also found appUcation in thin-layer chromatography. Th< reaction sequences that remain to be described were all designed to provide as speciflt a detection of the separated substances as possible. 

If the compound shows no convenient intrinsic feature, it may be possible to develop a detectable characteristic by chemical modification and, particularly in thin-layer chromatography and electrophoresis, many locating reagents which are specific for various groups of compounds are available. 

Kudzin ZH, Kotynski A, Kielbasinski P. 1991. Application of the iodine-azide reagent for selective detection of thiophosphoryl compounds in thin-layer chromatography. J Chromatogr 588 307-313. 

Bergstrom, G. and Lagercrantz, C., Diphenylpicrylhydrazyl as a reagent for terpenes and other substances in thin-layer chromatography, Acta Chem. Scand., 18, 560, 1964 Chem. Abs., 61, 2491h, 1964. 

Matthews, J.S., Steroids (CCXXIII) color reagent for steroids in thin-layer chromatography, Biochim. Biophys. Acta, 69, 163, 1963 Chem. Abs., 58, 14043d, 1963. 

W4. Waldi, D., Spray reagents for thin-layer chromatography. In Thin-Layer Chromatography. A Laboratory Handbook (E. Stahl, ed.), pp. 483-502. Academic Press, New York, 1965. 

Matthews, J. S. Steroids-CCXXIII. Color Reagent for Steroids in Thin-Layer Chromatography. Biochim. Biophys. Acta 69, 163 (1963). 

The various reactions applied for this piu pose can be classified into the same main types as the chromogenic reagents used to visualize sugars in thin-layer chromatography. In addition, there are certain reactions of other types that have been adapted for LC detection systems. The different kinds of reaction used are described and exemplified below. 

Although in situ) prechromatographic derivatization of the analytes in thin-layer chromatography (TEC) has been described in several cases, much more important are the postchromatographic reactions with various reagents. 

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