Rapid diagnostic methods

Several studies have established the utility of IHC as a sensitive, safe, and rapid diagnostic method for the diagnosis of viral hemorrhagic fevers such as yellow fever (Fig. 

Moreover, a rapid diagnostic method for analysing the three new cytokinins (1 MeZ, its 9-P-D-riboside and 2 deOZR) was developed to detect them in complex samples. The natural cytokinins and some of their above-mentioned derivatives and analogues, were characterised by fast atom bombardment tandem mass spectrometry (FAB MS MS). The protonated molecular ions of the examined cytokinins could be fingerprinted from breakdown patterns of their gaseous unimolecular... 

Scenarios of the future may be complicated by the possible use of multiple agents, or the delivery of chemical and replicating agents and/or their toxins that have been carefully matched, based on their stability and ability to generate specific symptoms. Health effects could be potentiated. Therefore, from a medical perspective, detection requires the availability of rapid diagnostic methods and procedures to assess illnesses that will be the result of multiple agents. 

Kudla, R. Small, R Huang, S. Rapid diagnostic method for distinguishing allergies and infections and nasal secretion collection unit. PCT Int. Appl. WO 2001098783, 2001 Chem. Abstr. 2001,136, 66584. 

Rapid Diagnosis - With the advent of antiviral drugs and their narrow spectrum of activity, it became apparent that rapid diagnostic methods were necessary for full use of these new agents. The classic virus identification systems are far too slow to be of value. One approach for the rapid diagnosis of a variety of viruses has been described (102). Another approach is that of fluorescent antibody (FA) which is rapid but highly specific (103,104). The FA technique is satisfactory... 

This chapter will provide an insight into the recent advances in rapid diagnostic methods for use in aquaculture, how appropriate these techniques are for this sector and a look towards future potential technologies. 

Table 5.1 Rapid diagnostic methods reported for the detection and identification of pathogens causing disease in finfish, molluscs and crustaceans...

LIGHTNER, D.V., R.M. REDMAN, D.w. MOORE, and M.A. PARK. 1993. Development and application of a simple and rapid diagnostic method to studies on hepatopancreatic parvovirus of penaeid shrimp. Aquaculture 116 15-23. 

MATSUMOTO, I., KAHURA, T., A new chemical diagnostic method for inborn errors of metabolism by mass spectrometry - rapid, practical, and simultaneous urinary metabolites analaysis, Mass Spec. Reviews, 1996,15,43-57. 

Radioimmunoassay. RIA caused a revolution in clinical diagnostics, providing a rapid, ultrasensitive method for the detection of nearly any agent to which an antibody could be developed. Sensitivity limits in the parts-per-trillion (pptr) or pg/mL are commonplace and RIAs are available for nearly every class of clinically relevant analyte including drugs, hormones, immunoglobulins, immune complexes, blood factors, microbes, viruses, and tumor antigens. 

As mentioned in Sect. 2.2.1.1 slow initiation is controlled either by the rate of ion-generation or cationation (cf. Eqs. (1)—(4). Based on earlier results [58,59] a convenient diagnostic method has been developed [1,29] to elucidate the nature of the rate determining step for rapid carbocationic polymerizations and in IMA (incremental monomer addition) experiments. For only one monomer addition, i.e., for AMI (all monomer in or for the first monomer addition in IMA) experiments, the relevant expressions are as follows ... 

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