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RAPD analysis

Leignel, V., Humbert, J.F. and Elard, L. (1997) Study by ribosomal DNA ITS2 sequencing and RAPD analysis on the systematics of four Metastrongylus species (Nematoda Metastrongyloidea). Journal of Parasitology 83, 606-611. [Pg.85]

Hosaka, K., Mori, M., Ogawa, K. (1994). Genetic relationships of Japanese potato cultivars assessed by RAPD analysis. Am. Potato J., 71, 535-546. [Pg.24]

J. Hammond and G. Spanswick, Biochem. Educ. 25, 109-111 (1997). A Demonstration of Genomic DNA Profiling by RAPD Analysis. ... [Pg.429]

Cunningham, C.O. and Mo, T.A. (1997) Random amplified polymorphic DNA (RAPD) analysis of three Norwegian populations of Gyrodactylus salaris (Monogenea Gyrodactylidae). Journal of Parasitology 83, 311-314. [Pg.134]

Fig. 2. Effect of DNA concentration and number of PCR cycles on RAPD analysis, shown on ethidium bromide-stained agarose gels, using template DNA from a single individual titmouse (Parus bicolor) and the 10-base primer AP5a4 (5 CTGTTGCTAC 3 ). (A) Various concentrations of template DNA amplified through 45 cycles of PCR. Lanes 1-11 contain 100, 50, 20, 10, 5, 2, 1, 0.1, 0.05, 0.01, and 0.005 ng of template DNA, respectively, in 25-/ Fig. 2. Effect of DNA concentration and number of PCR cycles on RAPD analysis, shown on ethidium bromide-stained agarose gels, using template DNA from a single individual titmouse (Parus bicolor) and the 10-base primer AP5a4 (5 CTGTTGCTAC 3 ). (A) Various concentrations of template DNA amplified through 45 cycles of PCR. Lanes 1-11 contain 100, 50, 20, 10, 5, 2, 1, 0.1, 0.05, 0.01, and 0.005 ng of template DNA, respectively, in 25-/<l reactions. Lane 12 contains no template DNA. (B) Constant amount of template DNA (0.6 ng/id) amplified with primer AP5a between 20 and 45 cycles. All reagents for the experiment were combined in a single tube, then aliquoted into twelve 25-/d reactions. Duplicate reactions were performed for each cycle length variation.
Fig. 3. RAPD analysis on an ethidium bromide-stained agarose gel using 0.6 ng// Fig. 3. RAPD analysis on an ethidium bromide-stained agarose gel using 0.6 ng//<l template DNA from several individual marsh wrens (Cistothorus palustris) and the 10-base primer AP4c4 (5 TCTCGATGCA 3 ). Both the gel photograph and a schematic of visible bands are shown.
Fig. 4. Typical RAPD analysis on an ettaidium bromide-stained agarose gel. Lane 1 contains DNA length standards. Lanes 2-11 contain 0.6 ng/ 1 each of genomic DNA from different individual marsh wrens (Cistothorus palustris) amplified with the 10-base primer AP5a4 (5 CTGTTGCTAC 3 ). Note that most amplified products lie between 0.6 and 2.3 kb, and there are about 8 to 10 well-defined PCR products per lane. Some products are mono-morphic in this population (M), whereas others are polymorphic (P). Amplification of all bands in lane 9 is weak, and testing of this individual should be repeated before polymorphic fragments are scored as present or absent. Fig. 4. Typical RAPD analysis on an ettaidium bromide-stained agarose gel. Lane 1 contains DNA length standards. Lanes 2-11 contain 0.6 ng/ 1 each of genomic DNA from different individual marsh wrens (Cistothorus palustris) amplified with the 10-base primer AP5a4 (5 CTGTTGCTAC 3 ). Note that most amplified products lie between 0.6 and 2.3 kb, and there are about 8 to 10 well-defined PCR products per lane. Some products are mono-morphic in this population (M), whereas others are polymorphic (P). Amplification of all bands in lane 9 is weak, and testing of this individual should be repeated before polymorphic fragments are scored as present or absent.
Currently, RAPD analysis is being used to study a wide variety of organisms. Successful amplifications have been obtained from mosquito... [Pg.307]

Barker GLA, Green JC, Hayes PK, Medlin LK (1994) Preliminary results using the RAPD analysis to screen bloom populations oiEmiliania huxleyi (Haptophyta). Sarsia 79 301-306... [Pg.214]

Jagadish, V., Robertson, J., and Gibbs, A., RAPD analysis distinguishes Cannabis sativa samples from different sources . Forensic Sci. Int., 79, 113-121 (1996). [Pg.72]

Halmschlager E, Messner R, Kowalski T, Prillinger H Differentiation of Ophiostoma piceae and Ophiostoma quercus by morphology and RAPD analysis. Syst Appl Microbiol 1994 17 554-562. Spatafora JW, Blackwell M Molecular systematics of unitunicate perithecial Ascomycetes The Clavicipitales-Hypocreales connection. Mycologia 1993 85 912-922. [Pg.287]

Zavaleta et al. (1997) and Reguant (2003) applied RAPD analysis, using different conditions, to evaluate intraspecific genetic diversity of O. oeni, and found that most strains showed unique RAPD patterns they proposed this method as a good tool to study the population dynamics of bacteria during MLF. [Pg.37]

SamarzijaD, Sikova S, Redzepovic S, Antunac N, Havranek J (2002) Application of RAPD analysis for identification of Lactococcus lactis subsp. cremoris strains isolated from artisanal cultures. Microbiol Res 157 13-17... [Pg.209]

Some restrictions limit the practical application of RAPD analysis. Dominance is the most important Umitation of RAPD. Dominant markers are not as discriminating as codominant markers to study population genetics. And as a result, more individuals must be sampled per loci for dominant markers (28). [Pg.278]

Recent study using RAPD analysis suggested A. rufa, not A. arguta, is involved in the parentage. [Pg.298]

See other pages where RAPD analysis is mentioned: [Pg.68]    [Pg.186]    [Pg.106]    [Pg.113]    [Pg.121]    [Pg.81]    [Pg.84]    [Pg.455]    [Pg.307]    [Pg.272]    [Pg.35]    [Pg.942]    [Pg.262]    [Pg.285]    [Pg.289]    [Pg.212]    [Pg.212]    [Pg.299]    [Pg.315]   
See also in sourсe #XX -- [ Pg.35 ]



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