Radioreceptor assays applications

Aaltonen L, Kanto J, Iisalo E, Pihlajamaki K (1984) Comparison of radioreceptor assay and radioimmunoassay for atropine pharmacokinetic application. Eur J Clin Pharmacol 26 613-617... 

L. Aaltonen, J, Kanto, E. lisalo, and K. Pihlajamaki, Comparison of radioreceptor assays and radioimmunoassay for atropine Pharmacokinetic application, Eur.. Clin. Pharmacol, 26 613-617 (1984). 

K Ensing, et al. Application of a radioreceptor assay in a pharmacokinetic study of oxitropium bromide in healthy volunteers after single IV, oral, and inhalation doses. Eur J Clin Pharmacol 37 507, 1989. 

J Schnabel, et al. Applications of scopolamine radioreceptor assay to pharmacokinetic studies in rats and humans. Clin Chem 36 1045, 1990. 

See also Radiochemical Methods Oven/iew Natural and Artificial Radioactivity Radionuclide Monitoring Technetium Radon Uranium Radiotracers Radio-Reagent Methods Radioreceptor Assays Gamma-Ray Spectrometry Food and Environmental Applications. 

See also Bioassays Overview. Capillary Electrophoresis Overview. Electrophoresis Oven/iew Principles Two-Dimensional Gels. Gas Chromatography Overview Mass Spectrometry. Immunoassays Overview. Immunoassays, Applications Food. Immunoassays, Techniques Radioimmunoassays Enzyme Immunoassays. Liquid Chromatography Reversed Phase Liquid Chromatography-Mass Spectrometry Food Applications. Mass Spectrometry Oven/iew Principles Matrix-Assisted Laser Desorption Ionization Time-of-Flight. Radiochemical Methods Radioreceptor Assays Food and Environmental Applications. Thin-Layer Chromatography Oven/iew. 

Radioreceptor assays have also been devised to measure blood levels of neuroleptics, p-receptor antagonists, and benzodiazepines. The development of these assays has an important clinical application since proper monitoring of blood levels is vital for maximizing therapeutic response, while minimizing toxicity. 

In the main this application deals with the analysis of plasma or semm for 25-OH-D3 and 1,25-(0H)2D3, two analytes which are present at low concentrations (25-OH-D3 1,25-(0H)2D3) in a relatively complex medium. It would appear at first sight that this presents a very difficult analytical challenge requiring the most extensive purification prior to quantitation. In the routine clinical laboratory, methods which are time-consuming and technically difficult are not as valuable as simpler and possibly, but not always, less specific methods. There is often a need in clinical situations for speed and simplicity and because of this, a considerable amount of development has taken place to produce simple and convenient assays in kit form, which depend upon the specificity of the saturation analysis quantitation procedure. The result is a method which couples solid-phase extraction with a radioreceptor assay which is both sensitive and specific. LC-MS meth-... 

Estimation of amino acid levels by radioreceptor binding techniques is a relatively new approach. The chapter on this topic reviews the underlying principles of ligand binding assays and details the practical considerations fundamental to the successful utilization of these techniques. Although this type of assay is extremely sensitive, it is beset with problems of specificity, and only one amino acid (GABA) can currently be determined accurately by the method Future developments should, however, extend its applicability to some of the other ammo acids, but it is likely to remain a method for determining a parhcular ammo acid, rather than one to be utilized for the measurement of a spectrum of ammo acids. 

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