Radioimmunoassay antigen competitive binding

Since the development of radioimmunoassay (RIA), many assays that rely on the specificity of the antigen-antibody binding reaction have been developed because of their inherent sensitivity and specificity. A typical competitive binding... 

Antibodies can have distinct advantages over the natural receptor molecules that are used in some competitive binding assays. They are stable, soluble proteins with known chemical and physical properties. - Procedures for their purification are available, " but usually diluted antisera is used in fluid phase radioimmunoassays. It has been estimated that an individual animal has the potential to produce antibodies specific for approximately 10 diverse immunodominant moieties. These molecules may possess binding constants for individual antigens on the order of... 

Radioimmunoassay is a competitive protein binding assay which utilizes an antibody as the binding protein. This assay also employs a highly purified antigen which has been radio-labeled (tagged). 

Part—VI has been solely devoted to Miscellaneous Assay Methods wherein radioimmunoassay (RIA) (Chapter 32) has been discussed extensively. Various arms of theoretical aspects viz., hapten determinants and purity importance of antigenic determinants and analysis of competitive antibody binding of isotopically labeled compounds. The applications of RIA in pharmaceutical analysis, such as morphine, hydromorphone and hydrocordone in human plasma clonazepam, flurazepam in human plasma chlordiazepoxide in plasma barbiturates, flunisolide in human plasma have been described elaborately. Lastly, the novel applications of RIA-techniques, combined RIA-technique-isotope dilution and stereospecificity have also been included to highlight the importance of RIA in the analytical armamentarium. 

The number of disadvantages outweigh the advantages of radioimmunoassays and this leads to development of non-radioisotopic immunoassay methods. Many substances can be firmly attached to the antibody without changes in binding properties towards the antigen. This principle has been used in some methods to be competitive with radioisotopic methods. In order for alternate methods to function as alternatives to radioisotopic... 

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