Rabbit thymus

Figure 1. Thin-layer chromatogram of rabbit thymus gangliosides. Plate developed with chloroform/methanol/0.5% CaClt 2H20 (55 45 10, by volume) spots located with resorcinol-HCl reagent. Total total gangliosides M monosialo-gangliosides D + T di- and trisialogan-gliosides.

According to more recent observations, cytallene (lid) is readily phosphorylated with ATP and 6,000-fold purified dCyd kinase from human leukemic spleen cells. In addition, cytallene (lid) but neither adenallene (11c) nor ddAdo inhibit phosphorylation of dCyd and dAdo catalyzed with dCyd kinase. The efficiency of phosphorylation of lid is lower than that of ddCyd. Results obtained with human T lymphoblastoid CEM cell line deficient in adenosine (Ado) kinase indicate that the latter enzyme may be important for phosphorylation of ddAdo. However, another study20 shows that ddAdo is not efficiently phosphorylated in a reaction catalyzed by Ado kinase from human thymus. It should also be noted that ddAdo is a substrate for dCyd kinase from the latter source whereas neither racemic adenallene (11c) nor / -enantiomer (31) inhibit phosphorylation of dAdo and dCyd catalyzed by the respective kinases from calf and rabbit thymus.21... 

Iwamori, M., and Nagai, Y, 1981, Comparative study on ganglioside compositions of various rabbit tissues. Tissue-specificity in ganglioside molecular species of rabbit thymus, Biochim. Biophys. Acta 665 214-220. 

Limb bud culture has been extensively studied in a variety of species, including rat, mouse, rabbit, chick and ferret. A review of the effects of antiviral drugs on limb bud and fetal thymus cultures has been published (Stahlmann et al., 1993). 

Figure 2. Sialic acid distribution in monosialoganglioside components of various rabbit tissues. Br brain Thy thymus Lu lung St stomach Li liver In intestine Ki kidney Te testis Mu muscle Column height shows percentage distribution of sialic acid in monosialoganglioside fraction. Numbers 1-6 unidentified monosialo-...

Comparison Of Thymus Gangliosides Of Various Animals. The unique characteristic of thymus ganglioside is its distinct species specificity. We examined thymus of human, calf, rat and mouse in addition to rabbit. As... 

There is a large species variation in A-acctylation. The hamster and rabbit possess relatively high A-acetylating capacities, while the dog has little or no acetylating capacity. Other species such as rats and mice are intermediate. A-Acetyl transferase activity has been demonstrated in liver, intestinal mucosa, colon, kidney, thymus, lung, pancreas, brain, erythrocytes, and bone marrow. A-Ethylmaleimidc, iodoacetate, and p-chloromercuribenzoate are irreversible inhibitors of the A-acetyl transferase. A variety of divalent cations such as Cu2+, Zn2+, Mn2+, and Ni2+ are also inhibitory. 

Fig. 1. An immuno-slot blot fin the detection of 0 -hydro]Q thy]deoxygiianosine. Calf thymus DNA was hydrm ethylated in vitro with 6.6 mAf of hydroxyethyl-nitrosourea. Aliquots containing 300, 100, 33.3, 11.1, 3.7, 1.2, and 0 finol of O -hydroxyethyldeoxyguanosine in 3 (ig ofheat-denatured DNA (corresponding to 217-0 pmol/tnol of deoxyguanosine) were blotted onto nitrocellulose and incubated with a rabbit anti-O -hydroxyethyldeoiQ guanosine serum (NPZ-146-2,1 15,000 see ref. 4). Bound antibodies were reacted with a goat-antirabbit IgG horseradish peroxidase conjugate and detected with hydrogen peroxide and 4-chloro-l-naphth6l.

Fig. 2. Typical densitometnc scan. Calf thymus DNA, methylated in vitro with 5 mM of methylnitrosourea, was denatured in 50 mM sodium hydroxide and blotted onto nitrocellulose. After incubation with a rabbit antiserum specific for O -methyldeoiQrguanosine (NPZ-193-1,1 8,000 see ref. 4) and alkaline phosphatase conjugated goat-antirabbit IgG, the blot was developed with 5-bromo-4-chloro-3-indolylphosphate toluidine salt and nitroblue tetrazolium chloride in diethanolamine buffer, as described in the Methods section, and scanned by reflectance densitometry at 530 nm. Slots contain 283, 94.7, 31.3, 10.4, 3.4, 1.1, and 0 fmol of 0 -methyl-deoxyguanosine, corresponding to 181-0.7 pmol/mol of deoxyguanosine.

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