Quantitative Bioanalysis—Selected Ion Monitoring

Pharmacokinetics Quantitative bioanalysis Selected ion monitoring Selected reaction monitoring Automated off-line solid-phase extraction Automated on-line extraction Fouda et al., 1991 Wang-Iverson et al., 1992 Covey et al., 1986 Kaye et al., 1992 Allanson et al., 1996 Simpson et al., 1998 Needham et al., 1998 

Long-term stability Degrad ant identification Standard method LC/MS protocol Volk et al., 1996 

The selection of a particular approach is discussed, with an emphasis on multiple stages of separation, chromatographic and mass, for providing unique capabilities and features for analysis. The applicability of these approaches to a wide variety of compounds of pharmaceutical interest is highlighted and compared to traditional quantitative analysis methods that use HPLC and GC/MS. 

Quantitative LC/MS assays generally involve four steps (1) sample preparation, (2) assay calibration, (3) sample analysis, and (4) data management. In this method, the human serum samples are prepared with a liquid-liquid extraction procedure. The internal 

Assay calibration involves the use of human serum samples fortified with CP-80,794 at 11 concentrations (six replicates per concentration) ranging from 0.05 to lOng/mL. In this particular case, due to a narrow linear dynamic range, two standard curves, ranging from 0.05 to lOng/mL, are constructed to provide the best accuracy. Serum blanks and an 11-point standard curve (two samples per concentration) are analyzed with each set of unknown samples. 

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