Quantitative Analysis of Cell Growth, Metabolism and Product Formation

Typical commercial cell culture systems include batch or fed-batch suspension reactors and perfused immobilized-cell reactors. However, the transient nature of batch culture causes difficulties in studying the effects of external stimuli on growth, metabolism and product formation. Due to metabolite concentration gradients, and the difficulty of obtaining representative cell samples, immobilized-cell reactors are also poorly suited for the analysis of cell growth and metabolism. As a result it is desirable to use well-defined model systems. Continuous-flow suspension reactors allow metabolic parameters to be measured at steady state, after cells have adapted to new (and possibly inhibitory) conditions. Perfusion reactors (with cells immobilized on suspended or stationary supports) extend these benefits to anchorage-dependent cells, and provide model systems for cell responses in vivo. However, while it is instructive to study the behaviour of cells under well-defined conditions, the results obtained must be verified in the culture system selected for commercial production. [Pg.133]

In this section methods are introduced for processing and presenting data for cell growth, metabolite consumption and product formation in a manner that facilitates [Pg.133]

Cell and Tissue Culture Laboratory Procedures in Biotechnology, edited by A. Doyle and J.B. Griffiths. 1998 John Wiley Sons Ltd. [Pg.133]

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