Pyrophosphatase and

Chau Y-P, Lu K-S. ZIO impregnation and cytochemical localization of thiamine pyrophosphatase and acid phosphatase activities in small granule-containing (SGC) cells of rat superior cervical ganglia. Histol Histopathol 1994 9 649-656. 

Fig. 3. Intracellular transport route of the SFV spike glycoproteins from the endoplasmic reticulum (ER), over the Golgi apparatus, to the plasma membrane (PM). The cis cisternae do not react positively for add phosphatase or thiamin pyrophosphatase, and do not label with ridn in thin frozen sections. The medial cisternae do not react positively for thiamin pyrophosphatase or acid phosphatase, but label with ridn. The trans cisternae are positive for all of these markers.

Zelinkova, Z., Deiijks, L. J., Stokkers, P. C., et al. (2006) Inosine triphosphate pyrophosphatase and thiopurine s-methyltransferase genotypes relationship to azathioprine-induced myelosuppression. Clin. Gastroenterol. Hepatol. 4, 44-49. 

Enzymes hydrolyzing UDP-glucuronic acid (UDP-glucuronic acid pyrophosphatase) and j8-D-glucuronosides ()8-glucuronidase) are present in microsomal material. Their activities in tissues depend on the species examined (G4, P2, W15). 

Lieberherr, M., Vreven, J., Vaes, G. The acid and alkaline phosphatases, inorganic pyrophosphatases and phosphoprotein phosphatase of bone. I. Characterization and assay. Bio-chim. Biophys. Acta 293, 160 (1973)... 

Majeska, R. J., Wuthier, R. E. Studies on matrix vesicles isolated from chick epiphyseal cartilage Association of pyrophosphatase and ATPase activities with alkaline phosphatase. Biochim. Biophys. Acta 391, 51 (1975)... 

Felix, R., Fleisch, H. Pyrophosphatase and ATPase of isolated cartilage matrix vesicles. 

After E. coli pyrophosphatase and 32Pj were mixed with the appropriate substrates and purified enzymes at pH 8.5, reduction of TPN+ proceeded with eventual trapping of 10% of the 3ZPi as thymidine triphosphate (TP3ZP3ZP). The net stoichiometry of the coupled reactions was 3ZP, —0.51 /xmole TP3ZP3ZP, +0.27 /xmole TDP-glucose, —0.26 /xmole and TPNH, +0.21 /xmole 12). 

In interesting recent studies, Poliak and co-workers (67, 68) have observed glucose-6-P phosphohydrolase, acid inorganic pyrophosphatase, and PPi-glucose phosphotransferase activities to be considerably higher in lipid-poor reticulosomes than in microsomal preparations. They hypothesized that reticulosomes, which they prepared by treatment of microsomal preparations from rat and chick livers with ribonuclease and deoxycholate (68), may be the precursors of endoplasmic reticulum, and... 

Subcellular Distribution op Glucose-6-P Phosphohydrolase, Inorganic Pyrophosphatase, and PPi-Glucose Phosphotransferase Activities op Human Liver ... 

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