Thiamine pyrophosphatase

Chau Y-P, Lu K-S. ZIO impregnation and cytochemical localization of thiamine pyrophosphatase and acid phosphatase activities in small granule-containing (SGC) cells of rat superior cervical ganglia. Histol Histopathol 1994 9 649-656. 

Fig. 3. Intracellular transport route of the SFV spike glycoproteins from the endoplasmic reticulum (ER), over the Golgi apparatus, to the plasma membrane (PM). The cis cisternae do not react positively for add phosphatase or thiamin pyrophosphatase, and do not label with ridn in thin frozen sections. The medial cisternae do not react positively for thiamin pyrophosphatase or acid phosphatase, but label with ridn. The trans cisternae are positive for all of these markers.

Roth, J. and Berger, E. G. 1982. Immunocytochemical localization of galactosyltransfer-ase in HeLa cells Codistribution with thiamine pyrophosphatase in trans-Golgi cistemae. J. Cell Biol 93, 223-229. 

Histochemical studies on Raillietina (.Raillietina) johri (Cestoda Davaineidae). II. Nucleoside diphosphatase and thiamine pyrophosphatase. Journal of Helminthology, 53 261-3. 

Murabe, Y. and Sano, Y., Thiamine pyrophosphatase activity in the plasma membrane of microglia, Histochemistry, 71,45, 1981. 

Nucleoside triphosphatase [Unspecific diphosphate phosphohydrolase] (3.6.1.15) is produced and catalyzes the reaction NTP + H2O = NDP + orthophosphate. [Also hydrolyses other nucleoside triphosphates, diphosphate, thiamin diphosphate and FAD.] m7G (5 ) pppN pyrophosphatase [7-Methylguanosine,... 

Sano, S., Matsuda, Y., and Nakagawa, H., 1988. Type B nucleoside-diphos-phatase of rat brain. Purification and properties of an enzyme with high thiamin pyrophosphatase activity. European Journal of Biochemistry. 171 231-236. 

Walski, M., and Gajkowska, B., 1998. Ultrastructural localization of thiamine pyrophosphatase in plasma membranes of brain phagocytes long time after cardiac arrest. Journal fur Hirnforschung. 39 183-191. 

It is likewise of equal interest that the Golgi apparatus is the site of many hydrolase activities such as alkaline and acid phosphatases, j8-glu-curonidase, nucleoside diphosphatase, and thiamine pyrophosphatase. 

However both can be cleaved by alkali to form UMP and the phosphate derivative of the carbohydrate. The pyrophosphate bond may also be split by a nucleotide pyrophosphatase from potatoes (338) or kidney (339). The enzyme is effective on DPN, DPNH, TPN, ADP, ATP, thiamine pyrophosphate, and FAD. 

Roth J Berger EG. Immunocytochemical localisation of galactosyltransferase in HeLa cells co-distribution with thiamine pyrophosphatase in trans-Golgi cisternae. J Cell Biol (1982) 93(1) 223-229. 

N-Acetylglucosamine transferase Other specific glycosyl transferases Thiamine pyrophosphatase... 

Meek, G. A., and Bradbury, S., 1963, Localization of thiamine pyrophosphatase activity in the Golgi apparatus of the mollusk. Helix aspersa, J. Cell Biol. 18 73. 

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