Pyrococcus juriosus

Section 4.3), which had to be replenished at every new cycle, the application of heat-stable polymerases from organisms such as Thermus aquations (Taq polymerase), Pyrococcus woesii (Pwo polymerase), Pyrococcus juriosus (Pfu polymerase), or Thermococcus litoralis (Vent polymerase) facilitated automation of the thermal cycling process. 

Blake, P.R., Lee, B., Summers, M.F., Adams, M.WW., Park, J.B., Zhou, Z.H. and Bax, A. (1992) Quantitative measurement of small through-hydrogen-bond and through space 1H-113Cd and 1H-199Hg J-couplings in metal-substituted rubredoxin from Pyrococcus Juriosus.J. Biomol. NMR, 2, 527—233. 

Fig. 11. Evolution of DNA polymerases. Upper panel distribution of DNA polymerases from A, B and C subfamilies between eubacteria, archaebacteria and eukaryotes. Lower panel alignments of regions 1 and 2a of E. coli DNA polymerase 11 (EcoPol II), Pyrococcus Juriosus (Pfii), Sulfolobus solfataricus (Sso), and human DNA polymerase-a (a-Hum). (Adapted from refs. [114,135-138].)...

As the importance of ion-pairs toward protein thermostability has been stressed in many cases, addition or removal of an ion-pair should have significant effects. A clear example is provided by mutagenesis studies of glutamate dehydrogenase from T kodakaraensis KOD1 (Tk-GDH)[361. The GDH from Pyrococcus Juriosus (Pf-GDH) and Tk-GDH are 83% identical in terms of primary structure. However, while Pf GDH displays a half-life of 12 h at 100 C, that of Tk-GDH is 4 h. The three-... 

The generation of 11.6 mol of Hi per mole of glucose-6-phosphate using enzymes of the oxidative pentose phosphate cycle coupled to a hydrogenase purified from Pyrococcus juriosus (Subtask C)... 

Practical applications of hydrogenase I from Pyrococcus Juriosus for NADPH generation and regeneration. J. Mol. Catal. B Enzym., 24-25, 39-52. 

TbADH ADH from Jhermoanaerobacter brodcu i /Hase I hydrt enase I from Pyrococcus Juriosus A. eutrophus Alcal enes eutrophus (whole c Us-containing Hase) C. oxydam Gluconobacter oxydans R. aOropha Ralstonia eutropha. 

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