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Pushing Expression Levels - Impact of Vector Design and Cell Clone Selection

Pushing Expression Levels - Impact of Vector Design and Cell Clone Selection [Pg.764]

Alternatively, sequences proposed to sta-bihze or increase expression may be inserted into the vector. Multiple such elements have been described such as ubiquitous chromatin opening (UCOE) element or the EASE element [23] US Patent 6,312,951). Comparable to matrix attachment regions, insulators or locus control regions (LCR), these elements act in cis [Pg.765]




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Cell design

Clone selection

Clones of cells

Cloning cells

Cloning of cells

Cloning selecting

Cloning selection

Cloning vectors

Design levels

Designer cells

Expression clone

Expression cloning

Expression vector

Level of Impact

Level of expression

PUSH

Pushing

Selection and Designation

Selection levels

Selective design

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