Purification workstations

Aurora Biomolecules dedicates to peptide synthesis (and polyclonal antibody production) for any small quantity purpose. FMOC chemistry (on Perceptive Biosystems Pioneer instruments) is used for peptides synthesis Online monitoring of the coupling efficiencies and HATU activation helps insure that the major component of the synthesis is the correct oligopeptide. Purification is firstly carried out by size exclusion chromatography, and then by HPLC on a PE vision purification workstation. Typically, 20 mg of pure peptide are obtained. The molecular weight of the purified peptide is determined as a final confirmation of quality. 

The degree of automations has literally revolutionized nucleic acid purification. The core of these systems are the automated liquid-handling workstations that involve the movement of multiple probes in Cartesian axes (x, y, z) over a deck configured with lab ware, such as microplates, tube racks, solvent reservoirs, washbowls, and disposable tips. They have the ability of aspirating and dispensing solvents from a source to a destination. Devices such as vacuum manifolds, heating blocks, and shakers have also been modified to handle 96-well (or more) plates. The multiprobe liquid handlers have a variable tip spacing that allows them to expand their tip-to-... 

The MS instrumentation is the most expensive part of the LC-MS system, hence efforts to improve the throughput of the LC-MS analysis often involve the use of parallel multiple columns that feed into a single mass spectrometer. Zeng and Kassel [99] developed an automated parallel analytical/preparative LC-MS workstation to increase the throughput for the characterization and purification of combinatorial libraries. The system incorporates two columns operated in parallel for both LC-MS analytical and preparative LC-MS purifications. A multiple-sprayer ESI interface was designed to support flows from multiple columns. The system is under complete software control and delivers the crude samples to the two HPLC columns from a single autosampler. The authors demonstrated characterization of more than 200 compounds per instrument per day, and purification of more than 200 compounds per instrument per night. De Biasi et al. [100] described a four-channel multiplexed... 

Preparation of 32 [39] The reaction set-up and product purification procedures were carried out using fhe Zymark Benchmate Robotic Workstation. 

Purification The workstation carried out the following steps for each tube ... 

The purification stop valves are normally open, motor-operated valves located inside containment. They close automatically on a low pressuriser level signal from the protection and safety monitoring system to preserve reactor coolant pressure boundary and to prevent uncovering of the heater elements in the pressuriser. However, they can also be manually operated from the main control room and the remote shutdown workstation (Section 9.3.6.3.7 of Reference 6.1). 

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