Pulping liquid atomization

Root powdering and demineralization. Sixty-five frozen and pulp-free tooth roots were ground in a Waring blender and powdered under liquid nitrogen in a freezer mill (Spex, Edison NJ, USA). The sieved powder (< 450 pm) was demineralized in dialysis bags in 0.5 M EDTA, pH 7.4, at 4°C with regular replacement of the solution. Demineralization was continued until no further calcium release could be detected by atomic absorption spectrometry. 

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