Pseudomonas savastanoi

Bertolini, E. Penyalver, R. Garcia, A. Olmos, A. Quesada, J. M. Cambra, M. Lopez, M. M. Highly sensitive detection of Pseudomonas savastanoi pv. savas-tanoi in asymptomatic olive plants by nested-PCR in a single closed tube./. Microbiol. Meth. 2003,52, 261-266. 

A water-soluble metabolite of indole-3-acetic acid is formed by certain strains of Pseudomonas savastanoi. The material has been isolated from culture filtrates of this organism and purified by adsorption on charcoal and by ion-exchange chromatography on sulfonic acid resins and quaternary ammonium resins (Hutzinger and Kosuge, 1968). The isolated metabolite was identified as indole-3-acetyl-e-L-lysine (CXII) by chemical means and by infrared and ultraviolet spectroscopy. 

I. The conversion of L-tryptophan to indole-3-acetamide by an enzyme system from Pseudomonas savastanoi. J. Biol. Chem. 241 3738-3744. 

Nucleotide sequences of Pseudomonas savastanoi indoleacetic acid genes show homology with Agrobacterium tumefaclens T-DNA. Proc. Natl. Acad. Sci., USA 82 6522-6526. 

Other cytokinins discovered in microoiganisms include trans-zeatin, /nmf-zeatin riboside, and 6-(4-hydroxy-l,3-dimethylbut-/ra. f-2-enylamino)-9-p-D-ribofuranosylpurine (4), a new cytokinin from the phytopathogenic bacterium Pseudomonas syringae pr. savastanoi, that causes olive knot and galls on stems of ash, jasmine, oleander, and privet. 

The unusual cytokinins 1 -methylzeatin and its riboside, both with a methyl group instead of hydrogen atom in C -position of the side chain, were identified in the plant pathogenic bacteria Pseudomonas syringae subsp. savastanoi and P. amygdali ([40] and references therein, [102]). These compounds have not yet been detected in plants and nothing is known about the path(s) of their formation. 

Indole 3-acetic acid (lAA) (17) also is found in certain bacteria. lAA is a virulence factor in the relationships of one bacterium. Pseudomonas syringae pv savastanoi, and its plant hosts, Nerium oleander (oleander, Apocynaceae) and Olea europaea (olive, Oleaceae). Virulence is assessed by the production of tumor-like outgrowths by the plants in response to secretion of phytohormones by the pathogen (Kosuge and Sanger, 1986). The bacterium produces lAA by conversion of L-tryptophan to indoleacetamide (18) and... 

Isolation and structural characterization of a-N-acetyl-indole-3-acetyl-e-L-lysine a new metabolite of indole-3-acetic acid from Pseudomonas syringae pv savastanoi. Phytochemistry 24 1499-1502. 

Figure 4. frfl/is-Zeatin (Pseudomonas syringae pv. savastanoi). 

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