Zeatin riboside

Other cytokinins discovered in microoiganisms include trans-zeatin, /nmf-zeatin riboside, and 6-(4-hydroxy-l,3-dimethylbut-/ra. f-2-enylamino)-9-p-D-ribofuranosylpurine (4), a new cytokinin from the phytopathogenic bacterium Pseudomonas syringae pr. savastanoi, that causes olive knot and galls on stems of ash, jasmine, oleander, and privet. 

In fact, kinetin was not the natural cell division hormone, although as an adenine derivative it is chemically closely related to the native kinins that have been isolated from plant sources. It was D. S. Letham in New Zealand (1967) who first succeeded with a crystalline cytokinin isolated from the milky stage of immature maize (Zea mays) kernels. It was 6-substituted iso-pentenylamino purine (N6-(A2-isopentenyl)) adenosine (Figure 2d). He called the substance zeatin, and zeatin riboside was, in fact, the major active constituent of coconut milk (Letham, 1974). 

Zeatin Zeatin riboside Sorghum bicolor partition PVP Sephadex-GlO reverse phase-C-0 lo UV-254 nm (58)... 

The isomeric (Z)-zeatine riboside was obtained from (22) by UV irradiation induced equilibration. 

Plant growth factors rra s-Zeatin Zeatin riboside ... 

Methy]zeatin riboside P. s. savastanoi Cl6H23Ns05 6-[(2 )-4-Hydroxy-l,3-dime thylbut-2-enyl -amino]-9-(3-D-ribofuranosyl)purine... 

Fig. lb. Cylokinins frans-Zeatin (t-Zeatin) Zeatin riboside Isopentenyl adenine (iPa) Kinetin Benzyladenine. 

Fig. 3. Reverse phase HPLC of a mixture of naturally-occurring cytokinins. Column 150x4.6 mm 5 p.m Spherisorb ODS-2. Mobile phase 30 min, 5-20% acetonitrile in water (pH 7.0 with triethylammonium bicarbonate). Flow rate 2 ml min. Detector absorbance monitor at 265 nm. Sample (1) adenine, (2) adenosine, (3) zeatin-7-glucoside, (4) zeatin-9-glucoside. (5) zeatin-O-glucoside, (6) zeatin, (7) dihydrozeatin-O-glucoside, (8) dihydrozeatin, (9) dihydrozeatin riboside-O-glucoside, (10) zeatin riboside, (11) dihydrozeatin riboside [6].

Anti-zeatinriboside Anti-cis- Anti-dihydro- Anti-isopentenyladenosine zeatin zeatin riboside riboside... 

Cytokinins.- In whole plants the natural cytokinins are zeatin and zeatin riboside. Some of the most used synthetic compounds are kinetin, thidiazuron (TDZ), benzyladenine (BA), among others. 

Zeatin riboside Benzyl adenine Zeatin-7-glucoside Napthaleneacetic acid Sodium docedyl sulphate Polyacrylamide gel electrophoresis Isoelectric focusing... 

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