Pseudomonas oleo vorans

Fluorescent pseudomonads are capable of synthesizing poly(3HAMCL)s from a large number of substrates. Work on the biotechnological production of poly(3HAMCL) has focused mainly on two model systems - Pseudomonas oleo-vorans and P. putida. P. oleovorans is able to use alkanes and alkenes as substrate due to the presence of the OCT-plasmid while P. putida, which does not have this plasmid, cannot. In contrast to P. oleovorans, however, P. putida can use carbohydrates such as glucose and fructose for the production of poly(3HAMCL). 

A. G. Katopodis, K. Wimalasena, J. Lee, and S. W. May, Mechanistic studies on non-heme monooxygenase catalysis Epoxidation, aldehyde formation, and demethylation by the omega-hydroxylation system of Pseudomonas oleo-vorans, J. Am. Chem. Soc., 206 7928 (1984). 

A rubredoxin-type protein of about 19,000 dalton, which binds up to two iron atoms per molecule (237, 238), has been found as a component of the co-hydroxylation system of the aerobic bacterium Pseudomonas oleo-vorans (213, 214). Its primary structure suggests two sequences homologous with that of rubredoxin from anaerobic bacteria (Fig. 11) (239). On this basis the authors have speculated on the possibility of an evolutionary divergence from a common ancestor for all these organisms so that the heavier type rubredoxin may have resulted by gene duplication. In the process, the specificity of the enzyme was so affected that 1-Fe rubredoxins cannot substitute for the 2-Fe protein in the co-hydroxylation reaction. 

Andujar M, Aponte MA, Diaz E, Schroder E (1997) Polyesters produced by Pseudomonas oleo-vorans containing cyclohexyl groups. Macromolecules 30 1611—1615 Angelova N, Hunkeler D (1999) Rationalizing the design of polymeric biomaterials. Trends... 

Scholz C, FuUer RC, Lenz RW (1994) Growth and polymer incorporation of Pseudomonas oleo-vorans on alkyl esters of heptanoic acid. Macromolecules 27 2886-2889 Scott G (1994) Environmental biodegradation of hydrocarbon polymers. In Doi Y, Fukuda K (eds) Biodegradable plastics and polymers. Elsevier, Amsterdam, pp 79-91 Scott G (1997) Abiotic control of polymer biodegradation. Trends Polym Sd 5 361-368 Scott G (1999) Biodegradable polymers. In Scott G (ed) Polymers and the environment. Royal Society of Chemistry, London, pp. 93-125 Scott G (2000) Green polymers. Polym Degrad Stab 68 1-7... 

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