Pseudocolored

Fig. 3 Optical slices (through 1 pm) of vegetative microspore of Equisetum arvense. Excitement with laser beams 488 (emission > 520 nm, green pseudocolor), 543 and 633 nm (fluorescence is observed at 650-750 nm, red pseudocolor).

The use of a sensitive camera system, such as a cooled charged-coupled device (CCD) camera, facilitates resolution and recording of the image, including the pseudocolors generated by probes 4-7. 

Fig. 12.15. Pseudocolor-scaled, three-dimensional macular pigment resonance Raman images of two volunteer subjects, along with related line plot profiles, derived for each distribution along nasal-temporal (solid line) and inferior-superior meridians (dashed line), both running through the center of the macula. Distribution...

In this review, we demonstrate that excellent IR spectra from microscopic regions of cells and tissue can be collected. These spectra are extremely sensitive to variations in the biochemical composition of the pixels from which the spectra were acquired. Multivariate analyses of the spectra datasets of cells, cell smears and tissue sections produce pseudocolor maps in a totally unsupervised fashion that reproduce the histopathology of tissue sections and cytological features of cells and cell smears. 

Figure 12.3 (a) A brightfield visible image of a com kernel section and (b) a corresponding pseudocolor... 

Pseudocolor images associated with photon emission are generated by the CCD image processor and transferred via video cable to a PCI frame grabber using the camera software (or equivalent). 

For co-localization of the bioluminescent photon emission, grayscale and pseudocolor images are merged using the appropriate software. 

Fig. 4.1. Frequency maps of two types of ischemic damage complete infarction (left) and scattered neuronal injury (right). Histology was obtained 24 h after left MCA occlusion in rats. Total number of animals was 9. Pseudocolor representation denotes the number of animals that showed the respective type of injury at this pixel. Note the widespread distribution of incomplete infarction over the affected left hemisphere. [Adapted from Alexis et al. (1996)]...

Figure 6 depicts five different labeled chromosome pairs, the crosstalk between channels is shown by the arrows in the top middle and bottom left images. Bottom right panel is a overlaid and pseudocolored image of the series. 

Fig. 18 Confocal micrographs of 24 h hypoxic H9C2 cardiocytes treated with rhodamine labeled IL (B) or rhodamine labeled PL (A). The micrographs are shown in pseudocolors. Cells treated with IL showed retention of membrane integrity and cell morphology (A). Liposomes represented as yellow colored regions are also discernable on the cells. Cells treated with PL showed presence of only dead cells with only a few cells having non-specifically attached PL (B). (From Ref...

HCA is a powerful method for data sorting based on local decision criteria. These criteria are based on finding the smallest distances between items such as spectra, where the term distances may imply Euclidean or Mahalanobis distances [17], or correlahon coefficients. HCA is, per se, not an imaging method, but can be used to construct pseudocolor maps from hyperspectral data sets collected from cells or tissue sections [18]. 

Figure 5.8 (a) Brightfield image (40x) of a stained urothelial cell (b) Pseudocolor image, obtained from Raman hyperspectral data via HCA, of the cell shown In (a) ... 

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