Pseudo-color

The lowest order Zemikes are tabulated in Table 1 along with their common optical name. The next figure shows fhe first 12 Zernike polynomials with a pseudo-color graphical representation of their value over a unit circle. In the plots, blue values are low and red values are high. 

Fig. 15. Temperature factors (B values) for DNA showtrrg areas of structure indetermirracy are represented in a pseudo-color model. Blue represents the lowest temperature factors and red the highest. Each gyre of the DNA is shown, ventral left, dorsal right. The temperature factor is one means of describing the atomic disorder. Sites indicated in red correlate with the holes shown in the previous figure and could be positions of HMGNl binding.

Fig.26 Pseudo-color time-resolved image of polystyrene nanobeads containing Pt porphyrin and conjugated to streptavidin immobilized to a biotinylated microarray surface (black teflon coated 96-well glass slide, spot diameter 1 mm, Erie Scientific) in different concentrations (25, 15, 10, 5, Ong streptavidin per well) [167]...

Although the human eye can only discriminate about 20 levels of intensity simultaneously, it can distinguish about 350,000 different shades of color. The significance of this is that pseudo color can be used in an image to convey details that would otherwise be lost in a gray scale image. 

Figure 11.4. A spotted DNA array with two-color detection of hybridization. An example of a spotted DNA array (16 X 20 elements) is shown after hybridization with two differentially labeled cDNA preparations, Cy3 (pseudo-colored green) and Cy5 (pseudo-colored red). The overlaying of the green and red images produces the image shown. The hue of each spot, ranging from green to red, indicates the relative expression level for the gene specific for each spot (image courtesy Packard Biochip Technologies). See color insert.

Fig. 4. Pseudo-color presentation of signals generated on the antibody microarray using dual color/reverse color experimental setup. Ten microgram of labeled protein per channel was incubated with two arrays using the dual color/reverse color setup. Red arrow on the first slide and green arrows on the second slide point out to some antigens with higher abundance in the heart sample, whereas green arrows on the first slide and red arrows on the second slide point out to some antigens with higher abundance in the liver sample (see Table 2).

Fig. 3.4 (a) The fluorescence images measured for an octadecanol-coated Au(l 11) electrode which was labeled with 2 mol% of both DilC,g(5) and 5-C,gFI. The images are pseudo-colored and combined to show the... 

Since humans can distinguish many more colors than shades of gray, another useful contrast stretching technique is to map the gray levels into colors this technique is called pseudo-color enhancement. 

Characterization of Microfiuidic Devices Using Microparticies, Fig. 5 (a) Fluorescent image (pseudocolor) taken under FITC filter cube showing streak of FITC-labeled 20 pm-diameter particles, (b) Fluorescent image (pseudo-color) taken under TRITC filter cube showing streak of TRITC-labeled 15 pm-diameter particles. (c) Stacked fluorescent image showing the successful... 

Mixing visualization using 1 gM fluorescein solution in mixer with breakup obstructions at increasing distances downstream. Fluorescein solution is flowing in the upper portion of the images (pseudo colored green) and water in the lower portion. Pixel intensity line scans across the microchannel cross-section at (b) the entrance and (c) 5 mm downstream [22]... 

Bright-field image Pseudo-color image Superimposed image... 

Fig. 6 (a) Fluorescence image of single DiD molecules embedded in a polystyrene film (5x5 xm ). The pseudo color scale shows the polarization direction of the fluorescence, indicating the molecular orientation (b) Fluorescence decay curves for one DiD molecule at different times. The red lines are the single-exponential functions fitted to the data (c) Time traces of fluorescence intensity and lifetime with 100 ms time interval. Reprinted with permission of [30], copyright (2004) American Chemical Society... 

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