Proteins strategy

Halobacterium salinarum Rapid expression Colorimetric expression Easy scale-up Cloning and transformation complicated Requires fusion protein strategy Lack of post-translational modifications... 

As a consequence of the various proinflammatory properties of S100 proteins, strategies targeting these molecules by in vivo administration of S100 antibodies are a novel option for anti-inflammatory therapies. Another approach is the inhibition of the release of these cytokine-like molecules at sites of inflammation. 

CALCINEURIN INHIBITORS AND THE GENERALIZATION OF THE PRESENTING PROTEIN STRATEGY... 

II. Inhibition by Immunophilin/Immunosuppressant Complexes The Presenting Protein Strategy... 

E. Y. Tshuva, S. J. Lippard, Synthetic models for non-heme carboxylate-bridged diiron metaUo-proteins Strategies and tactics, Chem. Rev. 104 (2004) 987. 

This chapter surveys new chemical methods for the attachment of synthetic molecules to proteins. Strategies targeting both native and unnatural functional groups are discussed, including an evaluation of the selectivity that each technique can achieve. A particular emphasis has been placed on the unique mechanistic attributes that these reactions possess and the practical circumstances under which they can be used. 

Han S, Le BV, Hajare HS, Baxter RHG, MUIct SJ (2014) X-Ray crystal structure of teicoplanin-A2-2 bound to a catalytic peptide sequence via the carrier protein strategy. J Oig Chem 79 8550-8556... 

Economou NJ, Nahoum V, Weeks SD, Gratsky KC, ZtaitnCT D, Townsend TM, Bhuiya MW, Cocklin S, Loll PJ (2012) A carrier protein strategy yields the stmctnre of dalbavancin. J Am Chem Soc 134 4637-4645... 

H. Saito, J. Mikami, S. Yamaguchi, M. Tanio, A. Kira, T. Arakawa, K. Yamamoto, S. Tuzi, Site-directed solid-state NMR studies on membrane proteins strategy and goals toward revealing conformation and dynamics as illustrated for C-labeled bacteriorhodopsin, Magn. Reson. Chem. 42 (2004) 218—230. 

In summary, many different cofactor recycling systems have been estabhshed. In vitro as well as in vivo systems display remarkable performance and they range from simple approach based on two single enzymatic to fusion-protein strategies. 

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