Protein Separation and Purification

Extensive research has been carried out into the molecular aspects of foreign protein production in whole plants to enhance the yield, quality and stability of the product and to facilitate protein separation and purification from the biomass [3, 6, 9], In contrast, comparatively little research has been undertaken to investigate the... 

At about the same time, the field of protein separation and purification was undergoing rapid development. The introduction of materials for protein chromatography, such as cross-linked dextran, agarose, and polyacrylamide, provided a means to study protein-surface interactions, as well as to dramatically advance knowledge in protein biochemistry. 

Shortcomings in current understanding of the thermodynamics of protein solutions can be illustrated by considering one of the oldest protein concentration methods, precipitation, and one of the newest and most technologically interesting methods for protein separation and purification, aqueous... 

Interactions between protein and polysaccharide have attracted increasing interests in the past two decades also because of their implications in many biological processes such as the organization of living cells and the use in many industrial applications such as microencapsulation, protein separation and purification, and in processed foods (Berdick and Morawetz 1954 Burgess and Carless 1984 Dubin et al. 1994 Tolstoguzov 1991). 

In electrophoresis, the net surface charge and the size of molecules separate them from one another in an electric field. Electrophoresis is one of the most effective methods of protein separation and purification. It has a very high resolving power to clearly separate charged protein molecules. This method of... 

Chang N, Hen SJ, and Klibanov AM. Protein Separation and Purification in Neat Dimethyl Sulfoxide. BiochemBiophysRes Communl991 176 1462-1468. 

Labrou NE. Dye-ligand affinity chromatography for protein separation and purification. In Walker JM, editor. Methods in molecular biology, affinity chromatography methods and protocols. Totowa, NJ Humana Press 2000. p. 129-39. 

Possible application of amphoteric gels for protein separation and purification purpose... 

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