Proteins producing biologically active

To date, complex proteins with biological activity (6), for use in X-ray crystal structure analysis (7) and ELISA systems (8), and for the development of animal drugs (9) have successfully been produced by using this system. Therefore, the Kaiko-baculovirus protein production system has broad applicability across the field of reverse chemical genetics for the analysis of protein function on the basis of interactions with chemical compounds. 

Bnrton, M., H. Nakai, P. Colosi, I Cnnnmgham, R. Mitchell, and L. Conto, Coexpression of factor VIII heavy and light chain adeno-associated viral vectors produces biologically active protein. Proc Natl Acad Sci USA, 1999. 96(22) 12725-30. 

Besides peptides, marine organisms have been reported to produce biologically active proteins, which are probably involved in the protection of organisms against physiological and stress conditions. Recently, these molecules have been cloned from sponges [324] and marine microorganisms [325],... 

Another chemical property relevant to druglike behavior is stereochemistry. These are molecules that contain a chiral center (a carbon with four different attachments) causing molecules with different chiral centers to be non-superimposable. These molecules present different three-dimensional arrays to proteins, and it is presumed that this controls biological potency and efficacy. For instance, the Easson-Stedman hypothesis proposes that a biologically active enantiomer interacts with at least three points on receptor to produce biological activity. Since three points... 

Panahi M, Alii Z, Cheng X, et al. Recombinant protein expression plasmids optimized for industrial E. coli fermentation and plant systems produce biologically active human insulin-like growth factor-1 in transgenic rice and tobacco plants. Transgenic Res., 2004 13(3) 245-259. 

Scapini P, Laudanna C, Pinardi C, Allavena P, Mantovani A, So2zani S, Cassatella MA Neutrophils produce biologically active macrophage inflammatory protein-3a (MIP-3c<)/CCL20 and MIP-3P/CCL19. Eur J Immunol 2001 31 1981-1988. 

Enzymes, proteins, antibodies, biologically active peptides and other protein derivatives have long been used in medicinal agents. Modem biotechnology opened horizons to create new processes and products. For example insulin, interferon and other medical proteins are now being produced commercially by genetic engineering. 

H)2-D3 is a weak agonist and must be modified by hydroxylation at position Cj for full biologic activity. This is accomplished in mitochondria of the renal proximal convoluted tubule by a three-component monooxygenase reaction that requires NADPFl, Mg, molecular oxygen, and at least three enzymes (1) a flavoprotein, renal ferredoxin reductase (2) an iron sulfur protein, renal ferredoxin and (3) cytochrome P450. This system produces l,25(OH)2-D3, which is the most potent namrally occurring metabolite of vitamin D. 

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