Protein-surface interactions thermo-responsive surfaces

On the other hand, cells and proteins exhibit space- and time-dependent dynamic changes in their conformations and functionalities in the human body. Since the 1990s, dynamic switchable surfaces have been comprehensively studied in order to control the biomolecular dynamics by modification with smart polymers [6], which show changes in their conformations and properties in response to external stimuli. Among them, thermo-responsive poly(AT-isopropylacrylamide) (PNlPA/ m) is one of the most successful models to control the interaction between biomolecules and the grafted substrates by temperature... 

Beyond the elastic modulus, SFM-based colloidal probe measuronents were successfully applied to study interaction forces between thermo-responsive polymers and protein-coated surfaces. This was shown by Cole, Voelcker, Thissen, Horn, and Griesser (2010) with bovine serum albumin-coated probes on PNiPAAm surfaces. Furthermore, time-dependent colloidal probe measuronents on thermo-responsive polymer coatings... 

In another study, QCM-D provides insight into how a protein interacts with a hydrogel film, e.g., monolayer or multilayer adsorption onto the surface versus penetration into the material. Alf et al. (2011a) show how bovine serum albumin penetrates an expanded thermo-responsive copolymer but forms an adsorbed monolayer when the material is collapsed above the phase transition temperature (Figure 6.11). 

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