Protein in children

Hidvegi, E., Cserhati, E., Kereki, E., Savilahti, E., and Arato, A. 2002. Serum immunoglobulin E, IgA, and IgG antibodies to different cow s milk proteins in children with cow s milk allergy Association with prognosis and clinical manifestations. Pediatr Allergy Immunol 13 255-261. 

Neither of the two physical methods is significantly affected by relative changes in albumin and y-globulin contents (R20, R30), but the extent of lipoid interference has not been clearly defined (D16). Comparative figures for serum proteins in children, obtained by the copper sulfate method, the Hitachi microrefractometer, and a biuret method (J12), are shown in Table 6, from which it is apparent that neither of the physical methods is appreciably affected by neutral-fat turbidity or intense icterus. Sunderman (855) also considered the relationship between serum protein concentration and refractivity not to be influenced by the cholesterol content of the serum. Nevertheless refractometry frequently overestimates protein in serum from patients with primary nephrotic syndrome (Table 6). 

Orotic acid is bound in the serum to proteins [436] and is metabolized in the liver [433]. The binding of orotic acid to serum proteins in children and adults receiving the drug orally at 80 mg/kg bodyweight per day for 28 days was less than 1% [437]. Maximal serum concentrations of orotic acid were attained 2-5 hours after intake and showed great individual variations, ranging from 0.8 to 10 xg per ml. On the other hand, the half-times for disappearance of orotic acid from the serum were about 1 hour and were more uniform [437]. The resorption of orotate was also followed in newborn infants [438], simultaneously with the level of enzyme activities in blood serum [439,440]. In connection with the anti-inflammatory effect of calcium orotate, [441] the calcium and phosphate metabolism in the treated patients was measured [442,443]. 

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