Protein identify peptides that bound target

Most one-bead-one-peptide libraries (see Section 4.3.7.3.1) are resin-bound and screened in direct solid-phase binding assays. In a typical binding assay, the library beads are incubated with the target protein labeled with a reporter molecule, such as an enzyme that catalyzes a reaction generating a colored compound, which in turn stains the beads to which the peptides that bind to the target protein are tethered. The positive (colored) beads are identified among the vast majority of noncolored beads, isolated, and submitted to structure determination of the peptides bound to them. 

---