Protein engineering transcription factors

Coiled-coil motifs have been known to play roles in conformational switching in natural proteins for some time (Oas and Endow, 1994). The key examples are influenza hemagglutinin (Bullough et al., 1994 Carr and Kim, 1993 Carr et al., 1997), and the heat shock transcription factor (Rabindran et al., 1993). Furthermore, an engineered form of GCN4-pl, with Asn-16 replaced by Ala, switches from dimer to trimer upon addition of... 

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