Protein database lysates

Wattiez R et al. Human bronchoalveolar lavage fluid protein two-dimensional database study of interstitial lung diseases. Electrophoresis 2000 21 2703-2712. Yanagida M et al. Matrix assisted laser desorption/ionization-time of flight-mass spectrometry analysis of proteins detected by anti-phosphotyrosine antibody on two-dimensional-gels of fibrolast cell lysates after tumor necrosis factor-alpha stimulation. Electrophoresis 2000 21 1890-1898. 

The expression levels of proteins in HeLa cells treated with 19 well-known inhibitors were successfully classified by cluster analysis according to the inhibitors mechanisms of action. Thus, we have since constructed a database from which expression data from separate experiments can be compared. Proteomic analyses of HeLa cells are performed after 18 h of exposure to test compounds at a concentration that inhibits cell growth by 80% or more. The expression data obtained from the around 300 spots, reproducibly detected in 2D-electrophoresis images of HeLa cell lysates, were used to calculate the ratio of each spot in treated and control cells and compare these ratios to the dataset in ChemProteoBase. 

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