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Protein cells, concentration

Whole blood is seldom used ia modem blood transfusion. Blood is separated into its components. Transfusion therapy optimizes the use of the blood components, using each for a specific need. Red cell concentrates are used for patients needing oxygen transport, platelets are used for hemostasis, and plasma is used as a volume expander or a source of proteins needed for clotting of the blood. [Pg.519]

Demaurex, N. and Frieden, M. (2003). Measurements of the free luminal ER Ca(2+) concentration with targeted cameleon fluorescent proteins. Cell Calcium 34, 109 19. [Pg.480]

In addition to the above extracellular parameters, cell concentration and cell activity are two important cell-associated parameters that determine how well a fermentation process is performing. The manufacturing of biological products (antibiotics, amino acids, monoclonal antibodies, and other protein products) at large scales requires that cells be cultured at high cell densities and stay metabolically active. Consequently, much effort has been expended to develop techniques that can allow the estimation of cell concentration and cell activity in real time during a fermentation. [Pg.418]

Menkes disease, an X-linked recessive condition, is caused by mutations in the gene encoding a Cu efflux protein. Cells from an affected individual accumulate high concentrations of Cu " that cannot be released from the cell. The symptoms result from functional Cu deficiency inasmuch as Cu absorbed from the intestine becomes trapped in the intestinal epithelial cells and delivery to other tissues is inadequate. [Pg.60]

As described elsewhere in this chapter, alterations in the activity of a number of lung enzymes have been described after acute and chronic ozone exposure. With the possible exceptions of the sulfhydryl-containing enzyme succinic dehydrogenase and the cytochrome P-4 en me benzopyrene hydroxylase, it is difficult to determine whether these findings are due to a direct oxidative effect of ozone or are secondary to changes in protein synthesis, concentrations of intermediates, or destruction of cells or organelles. [Pg.351]

An extremely important role of iron is the synthesis of haem for formation of erythrocytes and also for proliferating cells for synthesis of the mitochondrial enzymes that contain haem (e.g. cytochromes). The flux-generating enzyme in the synthesis of haem is aminolevulinic acid synthase (ALS) (Figure 15.20). If the cellular iron concentration is low, the concentration of this enzyme is increased in an attempt to maintain the rate of synthesis. As with the other two proteins, the concentration of ALS is controlled at the level of translation in a similar manner to that for transferrin, i.e. by increased stability of the mRNA, which is achieved by the binding of the IRP to the mRNA. [Pg.349]

Biochemical reactions are organized so that different reactions occur in different parts of the cell. This organization is most apparent in eukaryotes, where membrane-bounded structures are visible proof for the localization of different biochemical processes. For example, the synthesis of DNA and RNA takes place in the nucleus of a eukaryotic cell. The RNA is subsequently transported across the nuclear membrane to the cytoplasm, where it takes part in protein synthesis. Proteins made in the cytoplasm are used in all parts of the cell. A limited amount of protein synthesis also occurs in chloroplasts and mitochondria. Proteins made in these organelles are used exclusively in organelle-related functions. Most ATP synthesis occurs in chloroplasts and mitochondria. A host of reactions that transport nutrients and metabolites occur in the plasma membrane and the membranes of various organelles. The localization of functionally related reactions in different parts of the cell concentrates reactants and products at sites where they can be most efficiently utilized. [Pg.21]

Zhang, J.-G., Lindup, E. (1994) Cisplatin Nephrotoxicity Decreases in Mitochondrial Protein Sulphydryl Concentration and Calcium Uptake by Mitochondria from Rat Renal Cells, Biochem. [Pg.425]

Plasma half life varies considerably and is in the approximate range of 7 to 40 hours at doses above 100 mg, the half life is dose dependent. Phenytoin is widely and rapidly distributed throughout the body it is secreted in the milk, accumulates in red blood cells and amounts secreted in saliva show a linear relationship to non-protein bound concentrations in serum. [Pg.430]

Both the concentration-polarization layer and membrane fouling are present (filtration in presence of macromolecules, proteins, cells in the liquid phase). [Pg.322]

Fig. 2. Agonist-directed trafficking of 5-HT2C receptor stimulus. Concentration-response curves for 5-HT2C agonists measuring AA release (PLA2-AA) and IP accumulation (PLC-PI) in CHO-1C19 cells expressing the human 5-HT2c receptor (approx 200 fmol/mg protein). Cells, in serum-free medium, were labeled with 1 pCi/mL [3H]-myo-inositol (10-25 Ci/mmol) for 24 h and with 0.1 pCi/inL [14C]-arachidonic acid (57 mCi/mmol) for 4 h at 37°C. Measurements of PLC-mediated IP accumulation and PLA2-AA release were made from the same multiwell, simultaneously, after 10 min of... Fig. 2. Agonist-directed trafficking of 5-HT2C receptor stimulus. Concentration-response curves for 5-HT2C agonists measuring AA release (PLA2-AA) and IP accumulation (PLC-PI) in CHO-1C19 cells expressing the human 5-HT2c receptor (approx 200 fmol/mg protein). Cells, in serum-free medium, were labeled with 1 pCi/mL [3H]-myo-inositol (10-25 Ci/mmol) for 24 h and with 0.1 pCi/inL [14C]-arachidonic acid (57 mCi/mmol) for 4 h at 37°C. Measurements of PLC-mediated IP accumulation and PLA2-AA release were made from the same multiwell, simultaneously, after 10 min of...
Chlor-alkali production Electrochemical synthesis Water-organic liquid separation Organic liquid mixture separaion Fermentation products recovery and purification Cell harvesting, virus and antibody concentration Protein desalting, concentration and fractionation Blood processing, including artificial kidney Isolation, concentration, and identification of solutes and particulates... [Pg.379]


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See also in sourсe #XX -- [ Pg.309 ]




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Cells concentrators

Concentration cell

Protein concentrates

Protein concentration

Single-cell protein, concentrates

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