Propanediol dehydratase

PROPANEDIOL DEHYDRATASE PROPANEDIOL DEHYDRATASE Propanoyl-CoA, formation of, PROPIONYL-CoA SYNTHETASE Propionate CoA ligase,... 

Eagar, R. G. Jr., Bachovchin, W. W., Richards, J. H. (1975) Mechanism of action of adenosylcobalamin 3-Fluoro-1,2-propanediol as substrate for propanediol dehydratase-mechanistic implications, Biochemistry 14, 5523— 5528. 

Fig. 2. Mechanism of action of 5-deoxyadenosyl-cobalamin as the cofactor of propanediol dehydratase (EC 4.2.1.28). The mechanism is supported by the migration of from Cl of the substrate to C2 of the substrate and C5 of the cofactor. The same enzyme aiso dehydrates ethylene giycoi to acetaidehyde.

Active immobilized enzyme investigation of 441 a new means of immobilization Studies of the binding of cobaltamine and 393 the subunit interactions of propanediol dehydratase (Bia-dependent)... 

In industrial processes, 1,3-propanediol is used for the production of polyester fibers, polyurethanes and cydic compounds [85]. 1,3-Propanediol can be produced from glucose with the limiting step catalyzed by glycerol dehydratase. A metagenomic survey for glycerol hydratases from the environment resulted in seven positive clones, one of which displayed a level of catalytic efficiency and stability making it ideal for application in the produdion of 1,3-propanediol from glucose. 

Dunn-Coleman NS, Diaz-Torres M, Chase MW, Trimbur D (1998) Method for the recombinant production of 1,3-propanediol using protein x, protein 1, protein 2, and protein 3 for dehydratase reactivation. WO 98/21341... 

Schutz, H., Radler, F. (1984b). Propanediol-1,2-dehydratase and metabolism of glycerol of Lactobacillus brevis. Arch. Microbiol., 139, 366-370. 

Toraya et al. [60-63] used B3LYP (with the 6-311G(d) basis set) for calculations on the H-atom transfer steps in diol dehydratase reaction. Both H-atom transfers, i.e., from the substrate and re-abstraction of a hydrogen atom from 5 -deoxyadenosine, were considered. The models used in these studies included the substrate, 1,2-propanediol, a potassium cation found in the active site, and an ethyl radical as a mimic of the dAdo radical (Fig. 19.1). The activation barrier for the abstraction of the pro-S hydrogen atom of substrate by dAdo was calculated to be 9.0 kcal mol while the activation barrier for the reverse reaction between product radical and 5 -deoxyadenosine was 15.7 kcal moUk In the absence of the potassium cation the forward activation barrier is 9.6 kcal moU indicating that coordination of the substrate by the potassium cation has a minimal energetic effect on the H-atom transfer step, but seems to hold the substrate and intermediates in... 

Moreira AR, Ulmer DC, Linden JC (1981) Butanol toxicity in butylic fermentation. Biotechnol Bioengin Symp 11 567-579 Nagarajan V, Nakamura CE (1998) Production of 1,3-propanediol from glycerol by recombinant bacteria expressing recombinant diol dehydratase. US Patent 5821092... 

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