Promoters recombinant transcriptional activators

However, the use of recombinant transcriptional activators (RTAs) appears to be the most generally applicable system at present. The construction of RTAs is based on the modular structure of transcription factors, which allows for the combination of DNA binding and transactivation domains derived from different proteins. For example, RTAs have been used to establish a positive feedback loop initiated by transcription from a weak cell type-specific promoter [94], Such... 

A promoter to be used in E. coli should have certain characteristics to render it suitable for high-level production of recombinant protein. First, it must be strong, resulting in the accumulation of protein making up to 10-30% or more of the total cellular protein. Second, it should exhibit a minimal level of basal transcriptional activity. Third, promoters should be capable of induction in a simple and cost-effective manner. 

Kinoshita K, Harigai M, Fagarasan S, Muramatsu M, Honjo T A hallmark of active class switch recombination transcripts directed by I promoters on looped-out circular DNAs. Proc Natl Acad Sci USA 2001 98 12620-12623. 

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